Anti-MKRN1 antibody (ab72054)
Key features and details
- Rabbit polyclonal to MKRN1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MKRN1 antibody
See all MKRN1 primary antibodies -
Description
Rabbit polyclonal to MKRN1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human MKRN1 (C terminal).
Database link: Q9UHC7 -
Positive control
- WB: HeLa, Jurkat, HEK293T, TCMK-1, NIH/3T3; IP: HEK293T
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72054 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa). |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include FILIP1, p53/TP53, CDKN1A and TERT. Keeps cells alive by suppressing p53/TP53 under normal conditions, but stimulates apoptosis by repressing CDKN1A under stress conditions. Acts as a negative regulator of telomerase. Has negative and positive effects on RNA polymerase II-dependent transcription. -
Tissue specificity
Ubiquitous. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 4 C3H1-type zinc fingers.
Contains 1 RING-type zinc finger. -
Post-translational
modificationsAuto-ubiquitinated; which leads to proteasomal degradation. - Information by UniProt
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Database links
- Entrez Gene: 23608 Human
- Entrez Gene: 54484 Mouse
- Omim: 607754 Human
- SwissProt: Q9UHC7 Human
- SwissProt: Q9QXP6 Mouse
- Unigene: 728819 Human
- Unigene: 270484 Mouse
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Alternative names
- E3 ubiquitin-protein ligase makorin-1 antibody
- FLJ21334 antibody
- HGNC:7112 antibody
see all
Images
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All lanes : Anti-MKRN1 antibody (ab72054) at 0.04 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : HEK293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : TCMK1 cell lysate
Lane 5 : NIH3T3 cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 53 kDa
Exposure time: 10 secondsDetection: Chemiluminescence
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MGRN1 was immunoprecipitated from HEK-293T cell lysate (1.0 mg per IP reaction; 20% of IP loaded) with ab72054 at 6 µg per reaction. Western blot was performed from the immunoprecipitate with ab72054 at 0.1 µg/ml.
Lane 1: ab72054 IP in HEK-293T whole cell lysate.
Lane 2: ab72054 IP in HEK-293T whole cell lysate.
Lane 3: Control IgG IP in HEK-293T whole cell lysate.Detection: Chemiluminescence
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab72054 has been referenced in 4 publications.
- Xu Y et al. MiRNA-340-5p mediates the functional and infiltrative promotion of tumor-infiltrating CD8+ T lymphocytes in human diffuse large B cell lymphoma. J Exp Clin Cancer Res 39:238 (2020). PubMed: 33168024
- Liu M et al. Hypoxia-induced feedback of HIF-1a and lncRNA-CF129 contributes to pancreatic cancer progression through stabilization of p53 protein. Theranostics 9:4795-4810 (2019). PubMed: 31367258
- Panneer Selvam S et al. Binding of the sphingolipid S1P to hTERT stabilizes telomerase at the nuclear periphery by allosterically mimicking protein phosphorylation. Sci Signal 8:ra58 (2015). WB ; Human . PubMed: 26082434
- Cassar PA et al. Integrative genomics positions MKRN1 as a novel ribonucleoprotein within the embryonic stem cell gene regulatory network. EMBO Rep 16:1334-57 (2015). WB, Flow Cyt . PubMed: 26265008