• Product name
  • Description
    Rabbit polyclonal to MMP13
  • Host species
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Human recombinant MMP13 protein.

  • Positive control
    • Conditioned, serum-free medium from (TPA-treated) rat CCL-4 cells. Placenta tissue.



Our Abpromise guarantee covers the use of ab75606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 - 5 µg/ml. Predicted molecular weight: 54 kDa.
IHC-P Use a concentration of 10 - 20 µg/ml.


  • Function
    Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process.
  • Tissue specificity
    Seems to be specific to breast carcinomas.
  • Involvement in disease
    Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia Missouri type (SEMD-MO) [MIM:602111]. A bone disease characterized by moderate to severe metaphyseal changes, mild epiphyseal involvement, rhizomelic shortening of the lower limbs with bowing of the femora and/or tibiae, coxa vara, genu varum and pear-shaped vertebrae in childhood. Epimetaphyseal changes improve with age.
    Defects in MMP13 are the cause of metaphyseal anadysplasia type 1 (MANDP1) [MIM:602111]. Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia.
  • Sequence similarities
    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • Domain
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • CLG 3 antibody
    • CLG3 antibody
    • Collagenase 3 antibody
    • Collagenase3 antibody
    • MANDP1 antibody
    • Matrix metallopeptidase 13 (collagenase 3) antibody
    • Matrix Metalloproteinase 13 antibody
    • Matrix metalloproteinase-13 antibody
    • MMP 13 antibody
    • MMP-13 antibody
    • Mmp13 antibody
    • MMP13_HUMAN antibody
    see all


  • Anti-MMP13 antibody (ab75606) at 5 µg/ml + Conditioned, serum-free medium from (TPA-treated) rat CCL-4 cells

    Predicted band size: 54 kDa
    Observed band size: 54 kDa

  • ab75606 staining MMP13 in Rat shoulder tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked witha vector kit for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in antibody diluent) for 16 hours at 4°C. A undiluted Biotin-conjugated polyclonal was used as the secondary antibody.

    See Abreview


This product has been referenced in:
  • Schott EM  et al. Targeting the gut microbiome to treat the osteoarthritis of obesity. JCI Insight 3:N/A (2018). Read more (PubMed: 29669931) »
  • McDonald H  et al. Hexarelin treatment preserves myocardial function and reduces cardiac fibrosis in a mouse model of acute myocardial infarction. Physiol Rep 6:e13699 (2018). Read more (PubMed: 29756411) »

See all 24 Publications for this product

Customer reviews and Q&As

This antibody should detect the propeptide and the active form of MMP13. The pro form should be around 60KDa and the active form should be around 48kDa.

Thank you for submitting an Abreview of ab75606. As you may have noticed, your review has now been published on our website.
Since you are not satisfied with this antibody after using it in a tested species and application, we would like to follow...

Read More
Western blot
Mouse Tissue lysate - whole (Cerebellum Tumor Cell)
Loading amount
30 µg
Cerebellum Tumor Cell
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Oct 31 2012

Thank you for confirming those details.
I have now arranged for the replacement antibody (ab39012) to be sent to you. This is on the order number xxxxx. To check the status of the order please contact our Customer Service team and reference this n...

Read More

Thank you for sharing that extra information and sorry for the delay in getting back to you.
The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the t...

Read More

Thank you for providing those extra details.
May I ask a few further questions?
1. You mentioned the antigen retrieval you used trypsin and citrate buffer. Were these performed separately or together? Was any staining tried without antigen re...

Read More

Thank you once again for your enquiries which have been forwarded to me as my colleague Karin is away from the office today.

I notice you are still waiting for information regarding the antigen retrieval. We have managed to confirm that the ...

Read More

Thank you for contacting us and reporting the problems you have been encountering with the Anti-MMP13 antibody (ab75606).
I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and...

Read More

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

In or...

Read More

Expiration date: 19-08-2012

I am very pleased to hear you would like to accept our offer and test ab75606 in Hamster. This code will give you: 1 free [PRIMARY ANTIBODY/PROTEIN] OR VALUE OFF OF ORDER before t...

Read More

1-10 of 13 Abreviews or Q&A


Sign up