Anti-MMP3 antibody (ab137659)
Key features and details
- Rabbit polyclonal to MMP3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-MMP3 antibody
See all MMP3 primary antibodies -
Description
Rabbit polyclonal to MMP3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide, corresponding to a region within internal sequence amino acids 214-308 of Human MMP3
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Positive control
- 293T, A431, H1299, HeLaS3, Molt-4 and Raji cell lysate. HepG2 whole cell lysate (ab7900). HeLa cells, A549 xenograft tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137659 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 53 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 53 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase. -
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 4314 Human
- Omim: 185250 Human
- SwissProt: P08254 Human
- Unigene: 375129 Human
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Alternative names
- CHDS6 antibody
- Matrix metalloproteinase 3 antibody
- Matrix metalloproteinase 3 preproprotein antibody
see all
Images
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Anti-MMP3 antibody (ab137659) at 1/500 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 53 kDa
10% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded A549 xenograft labelling MMP3 with ab137659 at 1/500 dilution.
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Immunofluorescence analysis of methanol-fixed HeLa cells labelling MMP3 with ab137659 at 1/200 dilution. Lower panel is costained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab137659 has been referenced in 4 publications.
- Zhu L et al. MicroRNA-3662 targets ZEB1 and attenuates the invasion of the highly aggressive melanoma cell line A375. Cancer Manag Res 11:5845-5856 (2019). PubMed: 31388313
- Singh S et al. Interleukin-17 regulates matrix metalloproteinase activity in human pulmonary tuberculosis. J Pathol 244:311-322 (2018). IHC-P ; Human . PubMed: 29210073
- Maurel J et al. Phosphorylated-insulin growth factor I receptor (p-IGF1R) and metalloproteinase-3 (MMP3) expression in advanced gastrointestinal stromal tumors (GIST). A GEIS 19 study. Clin Sarcoma Res 6:10 (2016). PubMed: 27358721
- Wu Y et al. Effect of ß-anhydroicaritin on the expression levels of tumor necrosis factor-a and matrix metalloproteinase-3 in periodontal tissue of diabetic rats. Mol Med Rep 12:1829-37 (2015). Rat . PubMed: 25847066