Recombinant Anti-MSK1 (phospho S376) antibody [E375] (ab32190)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E375] to MSK1 (phospho S376)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MSK1 (phospho S376) antibody [E375]
See all MSK1 primary antibodies -
Description
Rabbit monoclonal [E375] to MSK1 (phospho S376) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: K562 cell lysate. IHC-P Human urinary bladder carcinoma. ICC/IF: HepG2 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E375 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32190 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/5000. Detects a band of approximately 87 kDa (predicted molecular weight: 90 kDa).
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IHC-P | (5) |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF | (1) |
1/500.
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Notes |
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WB
1/1000 - 1/5000. Detects a band of approximately 87 kDa (predicted molecular weight: 90 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/500. |
Target
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Function
Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14). -
Tissue specificity
Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 2 protein kinase domains. -
Post-translational
modificationsSer-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain. -
Cellular localization
Nucleus. Cytoplasm. Predominantly nuclear. Partially cytoplasmic. - Information by UniProt
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Database links
- Entrez Gene: 9252 Human
- Entrez Gene: 314384 Rat
- Omim: 603607 Human
- SwissProt: O75582 Human
- Unigene: 510225 Human
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Alternative names
- 90 kDa ribosomal protein S6 kinase 5 antibody
- EC 2.7.11.1 antibody
- KS6A5_HUMAN antibody
see all
Images
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All lanes : Anti-MSK1 (phospho S376) antibody [E375] (ab32190) at 1/2000 dilution
Lane 1 : HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor whole cell lysates
Lane 2 : HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor whole cell lysates with MSK1 (phosphor S376) peptide
Lane 3 : HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor whole cell lysates with MSK1 non-phospho peptide
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 90 kDa
Exposure time: 10 secondsBlocking/Diluting buffer: 5% NFDM/TBST
Observed band: 90, 80 kDa.
The 80 kDa band may represent isoform 2 or isoform 3 of MSK1. -
Immunocytochemistry analysis of HepG2 (human hepatocellular carcinoma epithelial cell) cells labeling MSK1 (phospho S376) with ab32190 at 1/500 (4 μg/mL). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. ab150077 AlexaFluor®488 Goat anti-Rabbit at 1/1000 (2 μg/mL) was used as the secondary antibody. DAPI (blue) was used as Nuclear counterstain.Confocal image showing decreased nuclear staining in HepG2 cells treated with Alkaline Phosphatase (37℃ 1h).
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All lanes : Anti-MSK1 (phospho S376) antibody [E375] (ab32190) at 1/2000 dilution
Lane 1 : Untreated HEK293 (human embryonic kidney) whole cell lysate
Lane 2 : HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor (EGF) whole cell lysate
Lane 3 : HEK293 (human embryonic kidney) cells were treated with Epidermal growth factor (EGF) whole cell lysate. Then the membrane was incubated with Alkaline phosphatase.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 90 kDa
Exposure time: 5 secondsBlocking/Diluting buffer 5% NFDM/TBST.
Observed band: 90, 80 kDa.
The 80 kDa band may represent isoform 2 or isoform 3 of MSK1. -
All lanes : Anti-MSK1 (phospho S376) antibody [E375] (ab32190) at 1/5000 dilution
Lane 1 : Untreated K562 cell lysate
Lane 2 : K562 cell lysate treated with Alkaline
Predicted band size: 90 kDa
Observed band size: 87 kDa why is the actual band size different from the predicted? -
Ab32190, at a dilution of 1/50, staining MSK1 in paraffin embedded human urinary bladder carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab32190 has been referenced in 4 publications.
- Li J et al. Programmable human histone phosphorylation and gene activation using a CRISPR/Cas9-based chromatin kinase. Nat Commun 12:896 (2021). PubMed: 33563994
- Khan SA et al. p38-MAPK/MSK1-mediated overexpression of histone H3 serine 10 phosphorylation defines distance-dependent prognostic value of negative resection margin in gastric cancer. Clin Epigenetics 8:88 (2016). PubMed: 27588146
- Rylander D et al. Pharmacological modulation of glutamate transmission in a rat model of L-DOPA-induced dyskinesia: effects on motor behavior and striatal nuclear signaling. J Pharmacol Exp Ther 330:227-35 (2009). PubMed: 19357321
- Westin JE et al. Spatiotemporal pattern of striatal ERK1/2 phosphorylation in a rat model of L-DOPA-induced dyskinesia and the role of dopamine D1 receptors. Biol Psychiatry 62:800-10 (2007). IHC ; Rat . PubMed: 17662258