Anti-MTAP antibody [2G4] (ab55517)
Key features and details
- Mouse monoclonal [2G4] to MTAP
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-MTAP antibody [2G4]
See all MTAP primary antibodies -
Description
Mouse monoclonal [2G4] to MTAP -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human MTAP. Immunogen based on GenBank: AAH18625.1.
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General notes
This product was changed from ascites to tissue culture supernatant on 30 April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: PBS -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
2G4 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab55517 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 31 kDa.
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ICC/IF |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 31 kDa. |
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the PNP/MTAP phosphorylase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 4507 Human
- Omim: 156540 Human
- SwissProt: Q13126 Human
- Unigene: 193268 Human
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Alternative names
- 5' methylthioadenosine phosphorylase antibody
- 5''-methylthioadenosine phosphorylase antibody
- BDMF antibody
see all
Images
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MTAP antibody (ab55517) at 1 μg/lane + HeLa cell lysate at 25 μg/lane.
This image was genrated using the ascites version of the product.
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ab55517 at 10 μg/ml staining MTAP in human Hella cells by Immunocytochemistry/ Immunofluorescence.
This image was genrated using the ascites version of the product.
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Overlay histogram showing HeLa cells stained with ab55517 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55517, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.
This image was genrated using the ascites version of the product.
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab55517 has been referenced in 3 publications.
- Limm K et al. Characterization of the Methylthioadenosine Phosphorylase Polymorphism rs7023954 - Incidence and Effects on Enzymatic Function in Malignant Melanoma. PLoS One 11:e0160348 (2016). WB . PubMed: 27479139
- Woollard WJ et al. Independent Loss of Methylthioadenosine Phosphorylase (MTAP) in Primary Cutaneous T-Cell Lymphoma. J Invest Dermatol 136:1238-46 (2016). PubMed: 26872600
- Czech B et al. Expression and function of methylthioadenosine phosphorylase in chronic liver disease. PLoS One 8:e80703 (2013). WB, IHC-P ; Human . PubMed: 24324622