Anti-Measles nucleoprotein antibody [2D7] (ab9882)
Key features and details
- Mouse monoclonal [2D7] to Measles nucleoprotein
- Suitable for: ELISA, WB
- Isotype: IgG1
Overview
-
Product name
Anti-Measles nucleoprotein antibody [2D7]
See all Measles nucleoprotein primary antibodies -
Description
Mouse monoclonal [2D7] to Measles nucleoprotein -
Host species
Mouse -
Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Measles virus -
Immunogen
Recombinant full length protein corresponding to Measles virus Measles nucleoprotein. Recombinant measles NP
-
Positive control
- recombinant measles NP
-
General notes
This product was changed from ascites to tissue culture supernatant on 28/11/2017. Please note that the dilutions may need to be adjusted accordingly.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.1% Sodium azide -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
2D7 -
Myeloma
NS0/1 -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9882 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ELISA |
1/1000 - 1/10000.
|
|
WB |
1/500 - 1/2000. Detects a band of approximately 52.5 kDa.
|
Notes |
---|
ELISA
1/1000 - 1/10000. |
WB
1/500 - 1/2000. Detects a band of approximately 52.5 kDa. |
Target
-
Relevance
Involved in the encapsidating of the genome in a ratio of 1 N per 6 ribonucleotides, resulting in the protection from nucleases. The nucleocapsid (NC) has an helical structure with either 12.35 or 11.64 N per turn, approximately 20nm in diameter, with a hollow central cavity approximately 5nm in diameter. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. During replication, encapsidation by N is coupled to RNA synthesis and all replicative products are resistant to nucleases. N is released in the blood following lysis of measles infected cells, it interacts then with human FCGR2B on immune cells, inducing apoptosis and blocking inflammatory immune response. Ntail binds to a protein on human thymic epithelial cells, termed Nucleoprotein Receptor (NR), inducing growth arrest -
Cellular localization
Virion. Host cytoplasm. -
Alternative names
- Measles nucleocapsid protein antibody
- N antibody
- NP antibody
- Nucleocapsid protein antibody
Images
-
Western blot of measles NP using monoclonal antibody 2D7 at a concentration of 0.5 µg/ml.
Lane 1 - prestained marker proteins
Lane 2 - recombinant measles NP.Western blot of measles NP using monoclonal antibody 2D7 at a concentration of 0.5 µg/ml.
Lane 1 - prestained marker proteins
Lane 2 - recombinant measles NP.
Datasheets and documents
-
SDS download
-
Datasheet download
References (5)
ab9882 has been referenced in 5 publications.
- Lo MK et al. GS-5734 and its parent nucleoside analog inhibit Filo-, Pneumo-, and Paramyxoviruses. Sci Rep 7:43395 (2017). PubMed: 28262699
- Morin I & Schaeffer PM Combining RNA-DNA swapping and quantitative polymerase chain reaction for the detection of influenza A nucleoprotein. Anal Biochem 420:121-6 (2012). PubMed: 21971444
- Morin I et al. IgG-detection devices for the Tus-Ter-lock immuno-PCR diagnostic platform. Analyst 136:4815-21 (2011). PubMed: 21980595
- Kurihara N et al. Contributions of the measles virus nucleocapsid gene and the SQSTM1/p62(P392L) mutation to Paget's disease. Cell Metab 13:23-34 (2011). IHC ; Human . PubMed: 21195346
- Zvirbliene A et al. Mapping of B cell epitopes in measles virus nucleocapsid protein. Arch Virol 152:25-39 (2007). PubMed: 16944047