Anti-Met Enkephalin antibody (ab22620)
Key features and details
- Rabbit polyclonal to Met Enkephalin
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Met Enkephalin antibody -
Description
Rabbit polyclonal to Met Enkephalin -
Host species
Rabbit -
Specificity
Recognizes methionine enkephalin in a wide range of species.
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Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Met Enkephalin conjugated to keyhole limpet haemocyanin (Glutaraldehyde). The conjugate was dialysed to remove excess conjugating agent prior to immunisation.
Sequence:YGGF M
Database link: P01210 -
Positive control
- Typical test tissues included rat and human brain, rat spinal cord, pig duodenum, guinea pig ileum (enteric nervous system) and human phaeochromocytoma (adrenomedullary tumour)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab22620 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/1000.
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IHC-P |
Use at an assay dependent concentration.
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Notes |
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ICC/IF
1/1000. |
IHC-P
Use at an assay dependent concentration. |
Target
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Function
Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(237-258) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum. -
Sequence similarities
Belongs to the opioid neuropeptide precursor family. -
Post-translational
modificationsThe N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 5179 Human
- Omim: 131330 Human
- SwissProt: P01210 Human
- Unigene: 339831 Human
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Alternative names
- Leu-enkephalin antibody
- Met-enkephalin antibody
- Met-enkephalin-Arg-Gly-Leu antibody
see all
Images
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ICC/IF image of ab22620 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22620, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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ab22620 (4µg/ml) staining met enkephalin in human testis using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic staining of seminal vesicles.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab22620 has been referenced in 2 publications.
- Lau BK et al. Mu-Opioids Suppress GABAergic Synaptic Transmission onto Orbitofrontal Cortex Pyramidal Neurons with Subregional Selectivity. J Neurosci 40:5894-5907 (2020). PubMed: 32601247
- Hollis F et al. Mitochondrial function in the brain links anxiety with social subordination. Proc Natl Acad Sci U S A 112:15486-91 (2015). IHC-Fr ; Rat . PubMed: 26621716