Mitochondrial Transmembrane Potential Apoptosis Detection Kit (ab65310)

Thank you for your reply.


Please assure your customer that the two are indeed the same product. Once we have sold the remaining units of this kit under ab65309, all stock will be listed under ab65310, with the option to purchase the 25 or 100 test size.


I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

You are correct, 1 ml is too much, and the instructions are too vague. You can pipet just a portion of that cell suspension onto a slide, for instance 50 ul, then coverslip. That will be 50,000 cells if you start with one million. If you find that the cells are too crowded on the slide, you can dilute the cell suspension with more buffer. Please let me know if you have more questions.

We assume this assay requires living cells, and that fixation before adding the dye will give unreliable results. However, we have not compared results from fixed and unfixed samples. The question is whether the fixation itself will alter the mitochondrial transmembrane potential. I am sorry we do not have an answer at this time.

Thank you very much for your call last week and for your patience while I have been in touch with the lab regarding your enquiry.

The new kit ab65310 actually contains the same reagents that were previously included in the kit ab65309. Ab65310 is just a larger size (100 reactions versus 25), so the results should be similar between these two kits. I'm not sure why this problem is only occuring with ab65310.

I apologize but the information in the troubleshooting section of the protocol is incorrect, and there is no "binding buffer" to use as a washing solution. The cells should be resuspended with the provided Incubation Buffer after incubation with the MitoCapture solution. The components of this Incubation Buffer are proprietary and we unfortunately can not disclose the composition of this reagent.

Since the cells are treated with a trypsin-like enzyme (and trypsin causes cell detachment), and apoptosis can also cause the cells to detach, it may not be possible to keep all the cells adhered to the surface. The lab scientists recommended very gentle treatment of the cells during the assay, however it may be necessary to collect the cells after apoptosis induction and perform the protocol on the cells in suspension as written, if they are still coming off of the surface.

I hope that this information will be useful, but if you have any further questions or comments please let me know and I'll be happy to help.