Overview

  • Product name

    Anti-Mitofusin 2 antibody [NIAR164]
    See all Mitofusin 2 primary antibodies
  • Description

    Rabbit monoclonal [NIAR164] to Mitofusin 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Mitofusin 2. The exact sequence is proprietary.
    Database link: O95140

  • Positive control

    • IHC-P: Human kidney tissue
  • General notes

    This antibody was developed as part of a collaboration between the National Institutes of Health and the lab of Paritosh Ghosh.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab124773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 86 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

ICC/IF 1/300.

For unpurified, use 1/100 - 1/250.

Target

  • Function

    Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression induces the formation of mitochondrial networks. Plays an important role in the regulation of vascular smooth muscle cell proliferation. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PARK2 recruitment to dysfunctional mitochondria. Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress. Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions.
  • Tissue specificity

    Ubiquitous; expressed at low level. Highly expressed in heart and kidney.
  • Involvement in disease

    Charcot-Marie-Tooth disease 2A2
    Neuropathy, hereditary motor and sensory, 6A
  • Sequence similarities

    Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. Mitofusin subfamily.
    Contains 1 dynamin-type G (guanine nucleotide-binding) domain.
  • Post-translational
    modifications

    Phosphorylated by PINK1.
    Ubiquitinated by non-degradative ubiquitin by PARK2, promoting mitochondrial fusion; deubiquitination by USP30 inhibits mitochondrial fusion.
  • Cellular localization

    Mitochondrion outer membrane. Colocalizes with BAX during apoptosis.
  • Information by UniProt
  • Database links

  • Alternative names

    • CMT2A antibody
    • CMT2A2 antibody
    • CPRP 1 antibody
    • CPRP1 antibody
    • EC 3.6.5.- antibody
    • Fzo antibody
    • HSG antibody
    • hyperplasia suppressor gene antibody
    • Hypertension related protein 1 antibody
    • KIAA0214 antibody
    • MARF antibody
    • MFN 2 antibody
    • Mfn2 antibody
    • MFN2_HUMAN antibody
    • Mitochondrial assembly regulatory factor antibody
    • Mitofusin-2 antibody
    • Mitofusin2 antibody
    • Transmembrane GTPase MFN2 antibody
    see all

Images

  • All lanes : Anti-Mitofusin 2 antibody [NIAR164] (ab124773) at 1/5000 dilution (purified)

    Lane 1 : mouse brain lysate
    Lane 2 : mouse kidney lysate
    Lane 3 : rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size: 86 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • All lanes : Anti-Mitofusin 2 antibody [NIAR164] (ab124773) at 1/5000 dilution (purified)

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HEK293 cell lysate
    Lane 4 : Raji cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size: 86 kDa
    Observed band size: 80 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Immunofluorescence staining of HEK293 cells with purified ab124773 at a working dilution of 1/300, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab124773 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

  • Immunohistochemical staining of paraffin embedded human kidney with purified ab124773 at a working dilution of 1/300. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Anti-Mitofusin 2 antibody [NIAR164] (ab124773) at 1/1000 dilution (unpurified) + Rat primary neurons cell lysate at 20 µg

    Secondary
    Anti-rabbit IgG HRP conjugate at 1/2000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 86 kDa


    Exposure time: 30 seconds

    See Abreview

  • Unpurified ab124773, at 1/50, staining Mitofusin 2 in formalin fixed paraffin embedded Human kidney tissue using immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

References

This product has been referenced in:

  • Yen JH  et al. Activation of dynamin-related protein 1 - dependent mitochondria fragmentation and suppression of osteosarcoma by cryptotanshinone. J Exp Clin Cancer Res 38:42 (2019). Read more (PubMed: 30691497) »
  • Kitaoka Y  et al. Effects of Nrf2 deficiency on mitochondrial oxidative stress in aged skeletal muscle. Physiol Rep 7:e13998 (2019). Read more (PubMed: 30756520) »
See all 25 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293R, HCT116)
Gel Running Conditions
Reduced Denaturing
Loading amount
35 µg
Specification
HEK293R, HCT116
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Aug 31 2019

Application
Western blot
Sample
Pig Tissue lysate - other (Mitochondria)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
20 µg
Specification
Mitochondria
Blocking step
Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Dec 19 2016

Application
Western blot
Sample
Rat Cell lysate - whole cell (Primary Neurons)
Loading amount
20 µg
Specification
Primary Neurons
Gel Running Conditions
Reduced Denaturing (4-12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted May 15 2013

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