Overview

  • Product name
    Mouse Angiopoietin 2 ELISA Kit
    See all Angiopoietin 2 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Mouse plasma 3 8.3%
    Inter-assay
    Sample n Mean SD CV%
    Mouse plasma 5 10.3%
  • Sample type
    Cell culture supernatant, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    17.3 pg/ml
  • Range
    68.75 pg/ml - 4400 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 94.5 67.4% - 120.9%
    Cell culture media 99.7 94.3% - 103.3%
    Heparin Plasma 112.4 81.4% - 143.2%
    EDTA Plasma 104.9 91.8% - 122.8%
    Citrate Plasma 94.7 87.1% - 103.3%

  • Assay time
    1h 30m
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Mouse, Rat
    Does not react with: Cow, Human
  • Product overview

    Abcam’s Angiopoietin 2 (ANG2) in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Angiopoietin 2 protein in mouse serum, plasma and cell culture supernatants samples.


     The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material.  TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.


     

  • Notes

    Angiopoietin 2 is a secreted glycosylated protein functioning in angiogenesis, inflammation and vascular development. Angiopoietin 2 binds to receptor tyrosine kinase TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signalling. Angiopoietin 2 can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, Angiopoietin 2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, Angiopoietin 2 may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab209883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • ELISA Protocol Summary
  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of Angiopoietin 2 were measured in duplicates, interpolated from the Angiopoietin 2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 6%, plasma (EDTA) 7.5%, plasma (heparin) 7.5%.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).  The mean Angiopoietin 2 concentration was determined to be 19,017 pg/mL in mouse serum, 2,802 pg/mL in rat serum, 31,192 pg/mL in mouse plasma (citrate), 26,451 pg/mL in mouse plasma (EDTA) and 33,857 pg/mL in mouse plasma (heparin).

Protocols

References

This product has been referenced in:
  • Lu W  et al. Transplantation of Rat Mesenchymal Stem Cells Overexpressing Hypoxia-Inducible Factor 2aImproves Blood Perfusion and Arteriogenesis in a Rat Hindlimb Ischemia Model. Stem Cells Int 2017:3794817 (2017). Read more (PubMed: 29238372) »
See 1 Publication for this product

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