• Product name
    Mouse Antithrombin III ELISA Kit (SERPINC1)
    See all Antithrombin III kits
  • Detection method
  • Precision
    Sample n Mean SD CV%
    Overall 4.4%
    Sample n Mean SD CV%
    Overall 9.4%
  • Sample type
    Cell culture supernatant, Urine, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    = 3 ng/ml
  • Range
    3.13 ng/ml - 100 ng/ml
  • Recovery

    96 %

  • Assay time
    4h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam’s Antithrombin III (SERPINC1) Mouse in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Antithrombin III concentrations in mouse plasma, serum, urine and cell culture supernatants.

    An Antithrombin III specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Antithrombin III specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Antithrombin III captured in plate.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform



Our Abpromise guarantee covers the use of ab108800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Standard curve : mean of duplicates (+/-SD) with backgrounds readings subtracted

  • Ms antithrombine III measured in biological fluids showing quantity (ng) per mL of sample tested



This product has been referenced in:
  • Foster DJ  et al. Advanced siRNA Designs Further Improve In Vivo Performance of GalNAc-siRNA Conjugates. Mol Ther 26:708-717 (2018). Read more (PubMed: 29456020) »
See 1 Publication for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A


Thank you for your phone call.
The standards for these kits are supplied in different masses. The mass for each vial will be printed on the label, and each lot will require a different volume of diluent in order to generate the 100 ng/mL standard. I hope this helps, please let me know if you need any additional information or assistance.

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Thank you for your patience.

Yes, I can confirm that the vial you received is 50x which is correct in this case - for the lot you received. We are looking into updating the datasheet. Going forward 80x will be the correct amount.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Thank you for contacting us.

I have contacted the corresponding department regarding your inquiry and hope to have an answer for you soon. I will be in touch.

I am sorryfor the confusion, and thank you for your patience.

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Thank you for contacting us. I have had a chance to speak with the lab regarding your question. Our data suggested that mouse serum ATIII has slightly higher concentration than plasma. We have about 200 – 600 ug/ml range of different mouse samples. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

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Thank you for contacting us. I was hoping that you might fill me in on a couple of points about your results with this assay. Have you been able to plot the standard curve accurately using the mean value of the triplicate readings for each standard and sample?  The minimum detectable dose of mATIII is typically 1ng/ml. What readings are you seeing with your sample?   Thank you in advance for getting this information for me. Please do not hesitate to contact us if you need any more advice or information.  

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