Overview

  • Product name
    Mouse CXCL16 ELISA Kit
    See all CXCL16 kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    RAW 264.7 5 6.3%
    Inter-assay
    Sample n Mean SD CV%
    RAW 264.7 3 3.2%
  • Sample type
    Cell culture supernatant, Serum, Citrate Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    7.6 pg/ml
  • Range
    31.25 pg/ml - 2000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 99.9 84.8% - 112.5%
    Cell culture media 96.7 86% - 108.3%
    Citrate Plasma 90.5 84.2% - 95.8%

  • Assay time
    1h 30m
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Mouse
    Does not react with: Rat, Rabbit, Goat, Guinea pig, Hamster, Cow, Dog, Human, Pig
  • Product overview

    Abcam’s CXCL16 in vitro SimpleStep ELISA™ (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CXCL16 protein in mouse serum, plasma and cell culture supernatant samples.

    The SimpleStep ELISA™ employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    CXCL16 is a chemokine. It induces a strong chemotactic response. It induces calcium mobilization. CXCL16 binds to CXCR6/Bonzo. CXCL16 also acts as a scavenger receptor on macrophages, which specifically binds to oxidized low density lipoprotein, suggesting that it may be involved in pathophysiology such as atherogenesis. CXCL16 is a plasma membrane protein.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate (12 x 8 well strips)

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab197744 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • ELISA Protocol Summary
  • Background-subtracted data values (mean +/- SD) are graphed.

  • RAW 264.7 cells were 24 hour serum starved and continued to culture either in the absence or in the presence of 5 µg/mL lipopolysaccharides (LPS) for 48 hours. Two dilutions (as indicated) of the cell culture supernatant samples were analyzed with this kit. Interpolated concentrations of CXCL16 adjusted for sample dilution are graphed in pg of CXCL16 per mL of supernatant (mean +/- SD, n = 2).

  • Background-subtracted data values (mean +/- SD) are graphed.

  • RAW 264.7 cells were 24 hour serum starved and continued to culture in the presence of 5 µg/mL lipopolysaccharides (LPS) for 48 hours. Cell culture supernatant samples was measured in 2-fold dilution series. Background-subtracted data values (mean +/- SD, n = 2) are graphed.

  • Background-subtracted data values (mean +/- SD, n = 2) are graphed.

Protocols

References

ab197744 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab197744.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
For licensing inquiries, please contact partnerships@abcam.com

Sign up