Mouse DLL4 ELISA Kit (ab213860)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 15.6 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse DLL4 ELISA Kit
See all DLL4 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 40pg/ml 2.44 = 6.1% 2 16 244pg/ml 18.78 = 7.7% 3 16 515pg/ml 36.56 = 7.1% Inter-assay Sample n Mean SD CV% 1 24 40pg/ml 2.64 = 6.6% 2 24 242pg/ml 20.81 = 8.6% 3 24 520pg/ml 38.48 = 7.4% -
Sample type
Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 10 pg/ml -
Range
15.6 pg/ml - 1000 pg/ml -
Assay time
3h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
The Mouse DLL4 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213860) is designed for the quantitative detection of Mouse DLL4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from rat specific for DLL4 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: S28-P525) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for DLL4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse DLL4 amount of sample captured in plate.
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Notes
Delta like ligand 4 is a protein that in humans is encoded by the DLL4 gene. It is mapped to 15q15.1. This gene is a homolog of the Drosophila delta gene. DLL4 is a transmembrane ligand for Notch receptors that shows restricted expression to endothelial cells (ECs), in particular to arteries and capillaries, and is involved in vascular development, and it also appeared to be a major trigger of Notch receptor activities previously implicated in arterial and vascular development. Mouse DLL4 could activate mouse Notch1 and mouse Notch4. DLL4 acts as a negative regulator of tumor angiogenesis, its blockade results in the striking uncoupling of tumor growth from vessel density, presenting a novel therapeutic approach even for tumors resistant to anti-VEGF therapies. In addition to it, this gene also plays an important role in promoting Th17 effector activity during mycobacterial challenge.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Adhesive Plate Seal 4 units Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Mouse DLL4 coated Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti- Mouse DLL4 antibody 1 x 100µl Lyophilized recombinant Mouse DLL4 standard 2 vials Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
Research areas
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Function
Plays a role in the Notch signaling pathway. Activates Notch-1 and Notch-4. -
Tissue specificity
Expressed in vascular endothelium. -
Sequence similarities
Contains 1 DSL domain.
Contains 8 EGF-like domains. -
Domain
The Delta-Serrate-Lag2 (DSL) domain is required for binding to the Notch receptor. -
Post-translational
modificationsUbiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- AOS6
- Delta 4
- delta 4 precursor
see all -
Database links
- Entrez Gene: 54485 Mouse
- SwissProt: Q9JI71 Mouse
- Unigene: 143719 Mouse
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab213860 has been referenced in 2 publications.
- Mukherjee P et al. Oxidized phospholipids cause changes in jejunum mucus that induce dysbiosis and systemic inflammation. J Lipid Res 63:100153 (2022). PubMed: 34808192
- Miranda K et al. MicroRNA-30 modulates metabolic inflammation by regulating Notch signaling in adipose tissue macrophages. Int J Obes (Lond) N/A:N/A (2018). PubMed: 29899524