Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (ab91361)

Overview

  • Product name
    Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control
    See all Mouse isotype controls
  • Tested applications
    Suitable for: Flow Cytmore details
  • Immunogen

    Synthetic peptide. Hapten from sequence of plant origin.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Purified from supernatant from an in vitro culture.
  • Clonality
    Monoclonal
  • Clone number
    B12/8
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas
  • Alternative names
    • Mouse Isotype Control

Applications

Our Abpromise guarantee covers the use of ab91361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab91361 is a negative isotype control

Images

  • Flow cytometry analysis of normal whole blood sample labeling IgG2a with ab91361 at 1μg. 

  • Overlay histogram showing A549 cells stained with ab60720 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab60720, 0.5µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

References

This product has been referenced in:
  • Rios DA  et al. Chronic hepatitis C liver microenvironment: role of the Th17/Treg interplay related to fibrogenesis. Sci Rep 7:13283 (2017). Read more (PubMed: 29038590) »
  • Khan A  et al. Mesenchymal stem cells internalize Mycobacterium tuberculosis through scavenger receptors and restrict bacterial growth through autophagy. Sci Rep 7:15010 (2017). Read more (PubMed: 29118429) »
See all 5 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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