Overview

  • Product name
    Mouse Periostin ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type
    Cell culture supernatant, Serum, Heparin Plasma, EDTA Plasma
  • Assay type
    Sandwich (qualitative)
  • Sensitivity
    0.05 ng/ml
  • Range
    0.05 ng/ml - 16 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 116.6 88% - 145%
    Plasma 109 84% - 134%
    Cell culture media 107 91% - 121%

  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    Abcam's Periostin Mouse ELISA Kit (ab193727) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Periostin in serum, plasma (Plasma sample should be collected using EDTA or heparin as an anticoagulant. Citrate is not recommended.) and cell culture supernatants.

    This assay employs an antibody specific for mouse Periostin coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures Periostin present in the samples. The wells are washed and biotinylated anti-mouse Periostin antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of Periostin bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate (12 x 8 well strips)

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    300X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated mouse Periostin detection antibody (lyophilized) 2 vials
    mouse Periostin Standards (lyophilized) 2 vials
    Pre-coated mouse Periostin Microplate (12 strips x 8 wells) 1 unit
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Function
    Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX (By similarity). Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization.
  • Tissue specificity
    Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus, thyroid tissue and breast. Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor. Overexpressed in breast cancers.
  • Sequence similarities
    Contains 1 EMI domain.
    Contains 4 FAS1 domains.
  • Post-translational
    modifications
    Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
  • Cellular localization
    Golgi apparatus. Secreted, extracellular space, extracellular matrix. Colocalizes with BMP1 in the Golgi.
  • Information by UniProt
  • Alternative names
    • OSF-2
    • Osteoblast-specific factor 2
    • Periostin
    • PN
    • POSTN
    • POSTN_HUMAN
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab193727 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

  • Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Protocols

References

ab193727 has not yet been referenced specifically in any publications.

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