Product nameMouse and Rabbit Specific HRP/DAB (ABC) Detection IHC kit
See all HRP/DAB detection kit kits
The reagents in this kit constitute a labeled streptavidin-biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen.
DAB is a suspected carcinogen. Handle with care.
Contains hydrogen peroxide.
Tested applicationsSuitable for: IHC-Pmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components 15 ml 15 ml 50x DAB Chromogen 1 x 0.5ml 1 x 0.5ml Biotinylated Goat Anti-Polyvalent 1 x 15ml 1 x 15ml DAB substrate 1 x 15ml 1 x 15ml Hydrogen Peroxide Block 1 x 15ml 1 x 15ml Protein block 1 x 15ml 1 x 15ml Streptavidin Peroxidase 1 x 15ml 1 x 15ml
RelevanceHRP/DAB detection kit is a immunoenzymatic antigen detection system for immunohistochemistry techniques.
Our Abpromise guarantee covers the use of ab64264 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent dilution.|
Immunohistochemical analysis staining CD163 in mouse soleus muscle section. Samples were incubated in rabbit CD163 antibody at 1:450 dilution and visualized using mouse and rabbit specific HRP/DAB detection IHC Kit (ab64264).
This product has been referenced in:
- Tong WY et al. Delivery of siRNA in vitro and in vivo using PEI-capped porous silicon nanoparticles to silence MRP1 and inhibit proliferation in glioblastoma. J Nanobiotechnology 16:38 (2018). Read more (PubMed: 29653579) »
- Nordquist E et al. Postnatal and Adult Aortic Heart Valves Have Distinctive Transcriptional Profiles Associated With Valve Tissue Growth and Maintenance Respectively. Front Cardiovasc Med 5:30 (2018). IHC-P . Read more (PubMed: 29740591) »