Overview

  • Product name
    Mouse c-Kit ELISA Kit (CD117)
    See all c-Kit kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 5 2.6%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 5.4%
  • Sample type
    Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    30 pg/ml
  • Range
    0.23 ng/ml - 15 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 94 92% - 94%
    Serum 118 115% - 120%
    Heparin Plasma 109 104% - 114%
    EDTA Plasma 105 93% - 144%
    Citrate Plasma 110 103% - 119%
    Mouse brain extract 104 103% - 106%

  • Assay time
    1h 30m
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Mouse
    Does not react with: Rat, Sheep, Rabbit, Goat, Guinea pig, Hamster, Cow, Dog, Human, Pig
  • Product overview

    Abcam’s c-Kit (CD117) in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of c-Kit protein in mouse serum, plasma, cell culture supernatant, cell and tissue extracts.

    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

    Sensitivity:
    Samples diluted in Sample Diluent NS – 62 pg/mL
    Samples diluted in 1X Cell Extraction Buffer PTR – 30 pg/mL

  • Notes

    c-Kit (CD117) is a member of the type III subfamily of receptor tyrosine kinases and is the cellular receptor for stem cell factor. Kit is essential for cell survival and proliferation, stem cell maintenance, mast cell development, melanogenesis, cellular migration, hematopoiesis, and gametogenesis. Mutations in human c-Kit are associated with gastrointestional stromal tumors, piebaldism, acute myelogenous leukemia, and mast cell disease.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate (12 x 8 well strips)

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse c-Kit Capture Antibody 1 x 600µl
    10X Mouse c-Kit Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent CPI 1 x 6ml
    Mouse C-Kit Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml
  • Research areas
  • Relevance
    KIT encodes the human homolog of the proto-oncogene c-kit. c-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism.
  • Cellular localization
    Cell Membrane; single-pass type I membrane protein
  • Alternative names
    • CD117
    • Mast/stem cell growth factor receptor precursor
    • PBT
    • Proto oncogene tyrosine protein kinase Kit
    • SCFR
    • v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog
    see all
  • Database links

Associated products

Applications

Our Abpromise guarantee covers the use of ab203361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • ELISA Protocol Summary
  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of c-Kit were measured in duplicate and interpolated from the c-Kit standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • The concentrations of c-Kit were measured in duplicate and interpolated from the c-Kit standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Interpolated values of c-Kit are plotted based on an extract load of 200 µg/mL.

Protocols

References

ab203361 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab203361.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up