Mouse liver tissue lysate - mitochondrial extract (ab110349)

Overview

  • Product name
    Mouse liver tissue lysate - mitochondrial extract
    See all Mouse Liver lysates
  • General notes

    Product was previously marketed under the MitoSciences sub-brand.

    2 mg of mouse liver mitochondria in 400ul of iso-osmotic buffer. This sample can act as a positive control in immunocapture applications and Blue Native PAGE.
    1 mg solubilized mitochondria for Immunocapture experiments. For the Immunocapture of OXPHOS complexes it is recommended that 180 µl of the mitochondrial membranes supplied be solubilized by the addition of 20 µl of 10% lauryl maltoside. Insoluble material is removed by centrifugation at 72,000g before immunocapture of complexes from the supernatant.

  • Tested applications
    Suitable for: SDS-PAGE, WBmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    Constituents: 0.02% PMSF, 0.16% Tris, 0.89% EDTA, 8.56% Sucrose
  • Concentration information loading...
  • Lysate notes
    2 mg of mouse liver mitochondria in 400ul of iso-osmotic buffer. This sample can act as a positive control in immunocapture applications and Blue Native PAGE.
    1 mg solubilized mitochondria for Immunocapture experiments. For the Immunocapture of OXPHOS complexes it is recommended that 180 µl of the mitochondrial membranes supplied be solubilized by the addition of 20 µl of 10% lauryl maltoside. Insoluble material is removed by centrifugation at 72,000g before immunocapture of complexes from the supernatant.

Applications

Our Abpromise guarantee covers the use of ab110349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
SDS-PAGE Use at an assay dependent dilution.
WB Use at an assay dependent dilution.

References

ab110349 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your phone call yesterday and for your patience while I have been in touch with the lab scientists at MitoSciences. Following is their reply to your questions: "These mitochondria were prepared by our mitochondrial isolation kit: https://www.abcam.com/Mitochondria-Isolation-Kit-for-Tissue-with-Dounce-Homogenizer-ab110169.html The principal : homogenize cells in an iso-osmotic buffer, centrifuge several times at low speed to remove cell debris, and nuclei.  Then spin at precisely 12000 g to collect most of the mitochondria. The mitochondria are washed and pelleted several more times at 12000 g.  Finally the washed pellet is taken up in an iso-osmotic buffer and frozen. This does a good job of mitochondrial isolation and enrichment, but there may be impurities there. It is possible to make very pure mitochondria by floating them on a  gradient of some kind (e.g percoll) but yields are lower so we do not do this. " Please let me know if you have any further questions and I'll be happy to help.  

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