Anti-Myc tag antibody [Myc.A7] (ab18185)
Key features and details
- Mouse monoclonal [Myc.A7] to Myc tag
- Suitable for: ELISA, Dot blot, WB, IP
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-Myc tag antibody [Myc.A7]
See all Myc tag primary antibodies -
Description
Mouse monoclonal [Myc.A7] to Myc tag -
Host species
Mouse -
Specificity
Recognizes overexpressed proteins containing Myc epitope tag fused to either amino- or carboxy-termini of targeted proteins in transfected mammalian cells. -
Tested applications
Suitable for: ELISA, Dot blot, WB, IPmore details -
Immunogen
Synthetic peptide:
EQKLISEEDL
, corresponding to amino acids 410-419 of Human Myc. -
Positive control
- WB: HEK-293 whole cell lysate ICC/IF: rat primary hippocampal neurons
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General notes
This product was changed from ascites to tissue culture supernatant on 25th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Protein A affinity chromatography -
Clonality
Monoclonal -
Clone number
Myc.A7 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab18185 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ELISA |
Use at an assay dependent concentration.
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Dot blot |
Use at an assay dependent concentration.
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|
WB | (1) |
1/1000.
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IP |
Use at an assay dependent concentration.
|
Notes |
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ELISA
Use at an assay dependent concentration. |
Dot blot
Use at an assay dependent concentration. |
WB
1/1000. |
IP
Use at an assay dependent concentration. |
Target
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Relevance
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. -
Cellular localization
Nuclear -
Alternative names
- c-myc tag antibody
- Myc Epitope Tag antibody
Images
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All lanes : Anti-Myc tag antibody [Myc.A7] (ab18185) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : MYC CRISPR-Cas9 edited HEK-293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : SH-SY5Y cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.False colour image of Western blot: Anti-Myc tag antibody [Myc.A7] staining at 1/1000 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab18185 was shown to bind specifically to Myc tag. A band was observed at 57 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in MYC CRISPR-Cas9 edited cell line ab256500 (CRISPR-Cas9 edited cell lysate ab263850). The band observed in the CRISPR-Cas9 edited lysate lane below 57 kDa is likely to represent a truncated form of Myc tag. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and MYC CRISPR-Cas9 edited HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution.
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All lanes : Anti-Myc tag antibody [Myc.A7] (ab18185) at 1/1000 dilution
Lane 1 : HEK293 lysate - untransfected
Lane 2 : HEK293 lysate - transfected with Myc-tagged protein
Protocols
Datasheets and documents
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Datasheet download
References (43)
ab18185 has been referenced in 43 publications.
- Na HJ et al. Cytosolic O-GlcNAcylation and PNG1 maintain Drosophila gut homeostasis by regulating proliferation and apoptosis. PLoS Genet 18:e1010128 (2022). PubMed: 35294432
- Gwak EJ et al. Mitochondrial ROS Produced in Human Colon Carcinoma Associated with Cell Survival via Autophagy. Cancers (Basel) 14:N/A (2022). PubMed: 35454800
- Zhang Y et al. Unique SMYD5 Structure Revealed by AlphaFold Correlates with Its Functional Divergence. Biomolecules 12:N/A (2022). PubMed: 35740908
- Parlak GC et al. The secondary pocket of cryptochrome 2 is important for the regulation of its stability and localization. J Biol Chem 298:102334 (2022). PubMed: 35933018
- Gul S et al. Discovery of a small molecule that selectively destabilizes Cryptochrome 1 and enhances life span in p53 knockout mice. Nat Commun 13:6742 (2022). PubMed: 36347873