Anti-Myc tag antibody (ab172)
Key features and details
- Chicken polyclonal to Myc tag
- Suitable for: WB, IP, ICC
- Isotype: IgY
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Myc tag antibody
See all Myc tag primary antibodies -
Description
Chicken polyclonal to Myc tag -
Host species
Chicken -
Tested applications
Suitable for: WB, IP, ICCmore details -
Immunogen
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide -
Concentration information loading...
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Purity
IgY fraction -
Purification notes
Antibodies were purified from the yolks using a proprietary method that yields a purity of >90%. Antibodies were affinity-purified over a SulfoLink® (Pierce) column conjugated with the peptide used for immunizations, and then dialyzed against PBS. Filter-sterilized (0.2 µm). -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab172 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/500.
|
IP |
1/250.
|
|
ICC |
1/250.
|
Notes |
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WB
1/500. |
IP
1/250. |
ICC
1/250. |
Target
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Relevance
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. -
Cellular localization
Nuclear -
Alternative names
- c-myc tag antibody
- Myc Epitope Tag antibody
Images
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All lanes : Anti-Myc tag antibody (ab172)
Lane 1 : Cultured normal mouse embryo fibroblasts were transfected with a plasmid containing the SAP1a cDNA fused with a c-myc epitope tag at its C-terminus. at 30 µg
Lane 2 : Cultured normal mouse embryo fibroblasts were transfected with a plasmid containing the SAP1a cDNA fused with a c-myc epitope tag at its C-terminus. at 20 µg
Lane 3 : Cultured normal mouse embryo fibroblasts were transfected with a plasmid containing the SAP1a cDNA fused with a c-myc epitope tag at its C-terminus. at 10 µg
Lane 4 : Cultured normal mouse embryo fibroblasts were transfected with a plasmid containing the SAP1a cDNA fused with a c-myc epitope tag at its C-terminus. at 5 µg
Protocols
Datasheets and documents
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Datasheet download
References (7)
ab172 has been referenced in 7 publications.
- Ma T et al. HOXA10 promotion of HDAC1 underpins the development of lung adenocarcinoma through the DNMT1-KLF4 axis. J Exp Clin Cancer Res 40:71 (2021). PubMed: 33596966
- Wan J et al. MYB70 modulates seed germination and root system development in Arabidopsis. iScience 24:103228 (2021). PubMed: 34746697
- Puri R et al. Mul1 restrains Parkin-mediated mitophagy in mature neurons by maintaining ER-mitochondrial contacts. Nat Commun 10:3645 (2019). PubMed: 31409786
- Czarna A et al. Single-cell analysis of the fate of c-kit-positive bone marrow cells. NPJ Regen Med 2:27 (2017). PubMed: 29302361
- Rutkowska-Wlodarczyk I et al. A proteomic analysis reveals the interaction of GluK1 ionotropic kainate receptor subunits with Go proteins. J Neurosci 35:5171-9 (2015). PubMed: 25834043
- Selak S et al. A role for SNAP25 in internalization of kainate receptors and synaptic plasticity. Neuron 63:357-71 (2009). ICC/IF . PubMed: 19679075
- Bréchet A et al. Protein kinase CK2 contributes to the organization of sodium channels in axonal membranes by regulating their interactions with ankyrin G. J Cell Biol 183:1101-14 (2008). ICC/IF . PubMed: 19064667