Anti-Myocilin antibody (ab41552)
Key features and details
- Rabbit polyclonal to Myocilin
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Rat, Human, Monkey
- Isotype: IgG
Overview
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Product name
Anti-Myocilin antibody -
Description
Rabbit polyclonal to Myocilin -
Host species
Rabbit -
Specificity
The antibody does not cross react with any other protein in RPE cells. -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Monkey -
Immunogen
Synthetic peptide:
CLVWDVGARTAQLRKANDQSGR
conjugated to KLH, corresponding to N terminal amino acids 25/46 of Myocilin -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab41552 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/200.
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|
WB |
Use at an assay dependent concentration.
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|
IP |
Use at an assay dependent concentration.
|
Notes |
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IHC-P
1/200. |
WB
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
Target
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Function
May participate in the obstruction of fluid outflow in the trabecular meshwork. -
Tissue specificity
Expressed in large amounts in various types of muscle, ciliary body, papillary sphincter, skeletal muscle, heart and other tissues. Expressed predominantly in the retina. In normal eyes, found in the inner uveal meshwork region and the anterior portion of the meshwork. In contrast, in many glaucomatous eyes, it is found in more regions of the meshwork and appeared more intensively than in normal eyes, regardless of the type or clinical severity of glaucoma. -
Involvement in disease
Defects in MYOC are the cause of primary open angle glaucoma type 1A (GLC1A) [MIM:137750]. Primary open angle glaucoma (POAG) is characterized by a specific pattern of optic nerve and visual field defects. The angle of the anterior chamber of the eye is open, and usually the intraocular pressure is increased. The disease is asymptomatic until the late stages, by which time significant and irreversible optic nerve damage has already taken place.
Defects in MYOC may also contribute to primary congenital glaucoma type 3A (GLC3A) [MIM:231300]. Defects in MYOC may contribute to this phenotype via digenic inheritance. GLC3A is an autosomal recessive form of primary congenital glaucoma (PCG). PCG is characterized by marked increase of intraocular pressure at birth or early choldhood, large ocular globes (buphthalmos) and corneal edema. It results from developmental defects of the trabecular meshwork and anterior chamber angle of the eye that prevent adequate drainage of aqueous humor. -
Sequence similarities
Contains 1 olfactomedin-like domain. -
Post-translational
modificationsDifferent isoforms may arise by post-translational modifications.
Glycosylated.
Palmitoylated. -
Cellular localization
Rough endoplasmic reticulum. Secreted. Cell projection > cilium. Located preferentially in the ciliary rootlet and basal body of the connecting cilium of photoreceptor cells, and in the rough endoplasmic reticulum. Also secreted. - Information by UniProt
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Database links
- Entrez Gene: 4653 Human
- Entrez Gene: 17926 Mouse
- Entrez Gene: 81523 Rat
- Omim: 601652 Human
- SwissProt: Q99972 Human
- SwissProt: O70624 Mouse
- SwissProt: Q9R1J4 Rat
- Unigene: 436037 Human
see all -
Alternative names
- GLC1A antibody
- GPOA antibody
- JOAG antibody
see all
Images
Datasheets and documents
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Datasheet download
References (11)
ab41552 has been referenced in 11 publications.
- Jasien JV et al. Anterior Segment Anatomy and Conventional Outflow Physiology of the Tree Shrew (Tupaia belangeri). Invest Ophthalmol Vis Sci 63:21 (2022). PubMed: 35040876
- Yoshida K et al. Extracellular matrix gene expression in human trabecular meshwork cells following mechanical fluid flow stimulation. Int J Ophthalmol 15:388-393 (2022). PubMed: 35310043
- Ghaffari Sharaf M et al. Towards preventing exfoliation glaucoma by targeting and removing fibrillar aggregates associated with exfoliation syndrome. J Nanobiotechnology 20:459 (2022). PubMed: 36303134
- Patterson-Orazem AC et al. Recombinant antibodies recognize conformation-dependent epitopes of the leucine zipper of misfolding-prone myocilin. J Biol Chem 297:101067 (2021). PubMed: 34384785
- Kasetti RB et al. Astragaloside IV Attenuates Ocular Hypertension in a Mouse Model of TGFβ2 Induced Primary Open Angle Glaucoma. Int J Mol Sci 22:N/A (2021). PubMed: 34830390