Mouse, Sheep, Human Predicted to work with:
Rat, Chicken, Chimpanzee
Unphosphorylated synthetic peptide:
, corresponding to amino acids 811-824 of Human N Cadherin (coupled to a carrier protein). This sequence is conserved in rat and mouse N cadherin, and has three amino acid differences from the conserved region in R Cadherin.
WB: Human endothelial cells (untreated or treated with pervanadate (1 mM) for 30 minutes) and mouse brain tissue.
This antibody gave a positive result in IHC in the following FFPE tissue: Human normal heart muscle.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 100 kDa.
Use a concentration of 1 µg/ml.
Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.
Western blot image of mouse brain lysate immunoprecipitated with no antibody (lane 1), anti-N-Cadherin (CP1751) rabbit polyclonal antibody (lane 2), and whole mouse brain lysate (lane 3). The blot was probed with anti-N-cadherin (Cytoplasmic) mouse monoclonal antibody (lanes 1-3) and detected using anti-Mouse Ig Light Chain specific:HRP secondary antibody.
Western blot - Anti-N Cadherin antibody (ab76057)This image is courtesy of an anonymous Abreview
All lanes : Anti-N Cadherin antibody (ab76057) at 1/1000 dilution
Lane 1 : MDA-MB-231 cells (Negative Control) Lane 2 : U2OS cells (Positive Control)
Lysates/proteins at 200000 cells per lane.
Secondary All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 100 kDa Observed band size: 100 kDa
IHC image of N Cadherin staining in Human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab76057, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Flow Cytometry - Anti-N Cadherin antibody (ab76057)This image is courtesy of an Abreview submitted by Zahra Abdalla
ab76057 staining N Cadherin in human BicR19 cells by Flow Cytometry. Cells were fixed with paraformaldehyde. The sample was incubated with the primary antibody (1/100) for 30 minutes. An undiluted Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. Gating Strategy: Oral epithelial cells.
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