Anti-N4BP1 antibody (ab106649)
Key features and details
- Rabbit polyclonal to N4BP1
- Suitable for: ICC, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-N4BP1 antibody
See all N4BP1 primary antibodies -
Description
Rabbit polyclonal to N4BP1 -
Host species
Rabbit -
Specificity
At least three isoforms of N4BP1 are known to exist; ab106649 will only detect the longest isoform. -
Tested applications
Suitable for: ICC, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
An 18 amino acid synthetic peptide near the N terminus of Human N4BP1 (NP_694574).
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Positive control
- WB: Jurkat and K562 whole cell lysates; ICC: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (stable for up to 12 months). -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106649 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use a concentration of 10 - 20 µg/ml.
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 100 kDa.
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Notes |
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ICC
Use a concentration of 10 - 20 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 100 kDa. |
Target
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Function
Inhibitor of the E3 ubiquitin-protein ligase ITCH. Acts by interacting with the second WW domain of ITCH, leading to compete with ITCH's substrates and impairing ubiquitination of substrates. -
Tissue specificity
Detected in heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis and ovary. -
Sequence similarities
Belongs to the N4BP1 family. -
Post-translational
modificationsMonoubiquitinated by NEDD4. Polyubiquitinated, leading to its degradation by the proteasome. Sumoylated by SUMO1, abrogating polyubiquitination and subsequent degradation. Desumoylated by SENP1, leading to accumulation in PML nuclear bodies.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus > nucleolus. Nucleus > PML body. Primarily localizes to the nucleolus. Also localizes to the PML nuclear bodies, when desumoylated. - Information by UniProt
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Database links
- Entrez Gene: 9683 Human
- SwissProt: O75113 Human
- Unigene: 511839 Human
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Alternative names
- FLJ31821 antibody
- KIAA0615 antibody
- MGC176730 antibody
see all
Images
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All lanes : Anti-N4BP1 antibody (ab106649) at 1 µg/ml
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 100 kDa
Exposure time: 30 secondsPrimary incubation for 1 hour at room temperature in 5% NFDM/TBST.
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Immunofluorescence of N4BP1 in HeLa cells with ab106649 at 20 ug/mL.
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Immunocytochemistry of N4BP1 in HeLa cells with ab106649 at 10 ug/mL.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab106649 has not yet been referenced specifically in any publications.