Anti-NCBP2 antibody (ab91556)
Key features and details
- Rabbit polyclonal to NCBP2
- Suitable for: IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NCBP2 antibody -
Description
Rabbit polyclonal to NCBP2 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IP: HeLa whole cell lysate IHC: human breast carcinoma tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab91556 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at 5-15 µg/mg of lysate.
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IHC-P |
1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IP
Use at 5-15 µg/mg of lysate. |
IHC-P
1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
NCBP2 is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. It has an RNP domain commonly found in RNA binding proteins, and contains the cap-binding activity. -
Cellular localization
Cytoplasmic and Nuclear -
Database links
- Entrez Gene: 510872 Cow
- Entrez Gene: 22916 Human
- Entrez Gene: 68092 Mouse
- Entrez Gene: 288040 Rat
- Omim: 605133 Human
- SwissProt: Q3ZBJ1 Cow
- SwissProt: P52298 Human
- SwissProt: Q9CQ49 Mouse
see all -
Alternative names
- 20 kDa nuclear cap binding protein antibody
- CBC2 antibody
- CBP20 antibody
see all
Images
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Lane 1 : Anti-NCBP2 antibody (ab91556) at 1 µg/ml (lot A302-552A)
Lane 2 : Anti-NCBP2 antibody (ab91556) at 1 µg/ml (lot A302-553A)
Lane 3 : Control IgG
All lanes : Hela whole cell lysate
Exposure time: 30 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling NCBP2 with ab91556 at 1/5000 (0.2µg/ml). Detection: DAB.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab91556 has been referenced in 2 publications.
- Bu H et al. Diagnostic and prognostic potential of the novel biomarker nuclear cap binding protein subunit 2 (NCBP2) in colon adenocarcinoma. J Gastrointest Oncol 13:1782-1792 (2022). PubMed: 36092349
- Shen Z et al. Translatome and Transcriptome Profiling of Hypoxic-Induced Rat Cardiomyocytes. Mol Ther Nucleic Acids 22:1016-1024 (2020). PubMed: 33294289