Anti-SPIN90 antibody (ab88467)
Key features and details
- Mouse polyclonal to SPIN90
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-SPIN90 antibody
See all SPIN90 primary antibodies -
Description
Mouse polyclonal to SPIN90 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant full length protein within Human SPIN90. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: NP_909119.1 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88467 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa).
|
Notes |
---|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 79 kDa (predicted molecular weight: 79 kDa). |
Target
-
Function
Has an important role in stress fiber formation induced by active diaphanous protein homolog 1 (DRF1). Induces microspike formation, in vivo (By similarity). In vitro, stimulates N-WASP-induced ARP2/3 complex activation in the absence of CDC42 (By similarity). May play an important role in the maintenance of sarcomeres and/or in the assembly of myofibrils into sarcomeres. Implicated in regulation of actin polymerization and cell adhesion. -
Tissue specificity
Highest expression in heart, brain, skeletal muscle, kidney and liver. Lower levels in placenta, lung, small intestine and leukocytes. Weak expression in colon, thymus and spleen. -
Involvement in disease
Note=A chromosomal aberration involving NCKIPSD/AF3p21 is found in therapy-related leukemia. Translocation t(3;11)(p21;q23) with MLL. -
Sequence similarities
Contains 1 SH3 domain. -
Cellular localization
Nucleus. Colocalizes with DRF1 at membrane ruffles, and with Nck at Z-disks in mature cardiac myocytes. - Information by UniProt
-
Database links
- Entrez Gene: 51517 Human
- Entrez Gene: 301009 Rat
- Omim: 606671 Human
- SwissProt: Q9NZQ3 Human
- Unigene: 655006 Human
-
Alternative names
- 54 kDa VacA-interacting protein antibody
- 54 kDa vimentin-interacting protein antibody
- 90 kDa SH3 protein interacting with Nck antibody
see all
Images
-
Anti-SPIN90 antibody (ab88467) at 1 µg/ml + rat brain tissue lysate at 50 µg
Secondary
Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
Predicted band size: 79 kDa
Observed band size: 79 kDa -
Anti-SPIN90 antibody (ab88467) at 1 µg/ml + HeLa cell lysate at 50 µg
Secondary
Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
Predicted band size: 79 kDa
Observed band size: 79 kDa -
All lanes : Anti-SPIN90 antibody (ab88467) at 1 µg/ml
Lane 1 : SPIN90 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
Predicted band size: 79 kDa
Observed band size: 79 kDa
Protocols
Datasheets and documents
-
Datasheet download
References (1)
ab88467 has been referenced in 1 publication.
- Wyse MM et al. Dia-Interacting Protein (DIP) Imposes Migratory Plasticity in mDia2-Dependent Tumor Cells in Three-Dimensional Matrices. PLoS One 7:e45085 (2012). ICC/IF ; Human . PubMed: 23024796