Overview

  • Product name
    Anti-NDRG1 antibody [EPR5592]
    See all NDRG1 primary antibodies
  • Description
    Rabbit monoclonal [EPR5592] to NDRG1
  • Host species
    Rabbit
  • Specificity
    The antibody could recognize NDRG2, NDRG3 and NDRG4. Please contact our Scientific Support team for further information.
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-P, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NDRG1 aa 50-150. The exact sequence is proprietary.

  • Positive control
    • Human colon tissue; fetal kidney and HeLa lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 48 kDa (predicted molecular weight: 43 kDa).
ICC/IF 1/100 - 1/250.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Use a HRP/AP polymerized secondary antibody.
IP Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      May have a growth inhibitory role.
    • Tissue specificity
      Ubiquitous; expressed most prominently in placental membranes and prostate, kidney, small intestine, and ovary tissues. Reduced expression in adenocarcinomas compared to normal tissues. In colon, prostate and placental membranes, the cells that border the lumen show the highest expression.
    • Involvement in disease
      Defects in NDRG1 are the cause of Charcot-Marie-Tooth disease type 4D (CMT4D) [MIM:601455]; also known as hereditary motor and sensory neuropathy Lom type (HMSNL). CMT4D is a recessive form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy and primary peripheral axonal neuropathy. Demyelinating CMT neuropathies are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. By convention, autosomal recessive forms of demyelinating Charcot-Marie-Tooth disease are designated CMT4.
    • Sequence similarities
      Belongs to the NDRG family.
    • Cellular localization
      Cytoplasm. Nucleus. Cell membrane. Whereas in prostate epithelium and placental chorion it is located in both the cytoplasm and the nucleus, nuclear staining is not observed in colon epithelium cells. Instead its localization changes from the cytoplasm to the plasma membrane during differentiation of colon carcinoma cell lines in vitro.
    • Information by UniProt
    • Database links
    • Alternative names
      • 42 kDa antibody
      • Anti GC4 antibody
      • cap43 antibody
      • cmt4d antibody
      • Differentiation related gene1 protein antibody
      • Differentiation-related gene 1 protein antibody
      • Drg 1 antibody
      • DRG-1 antibody
      • drg1 antibody
      • gc4 antibody
      • GC4 antibody antibody
      • hmsnl antibody
      • Human mRNA for RTP complete cds antibody
      • N myc downstream regulated gene 1 antibody
      • N myc downstream regulated gene 1 protein antibody
      • N-myc downstream-regulated gene 1 protein antibody
      • Ndr 1 antibody
      • ndr1 antibody
      • NDRG 1 antibody
      • Ndrg1 antibody
      • NDRG1 protein antibody
      • NDRG1_HUMAN antibody
      • Nickel specific induction protein antibody
      • Nickel specific induction protein Cap43 antibody
      • Nickel-specific induction protein Cap43 antibody
      • nmsl antibody
      • Nmyc downstream regulated antibody
      • Nmyc downstream regulated gene1 antibody
      • Nmyc downstream regulated gene1 protein antibody
      • Protein NDRG1 antibody
      • Protein regulated by oxygen 1 antibody
      • Protein regulated by oxygen1 antibody
      • Proxy1 antibody
      • Reduced in tumor antibody
      • Reducin antibody
      • Reducing agents and tunicamycin responsive protein antibody
      • Reducing agents and tunicamycin-responsive protein antibody
      • Rit42 antibody
      • RTP antibody
      • targ1 antibody
      • TDD5 antibody
      • tdds antibody
      • Tunicamycin responsive protein antibody
      see all

    Images

    • All lanes : Anti-NDRG1 antibody [EPR5592] (ab133572) at 0.0484 µg/ml (unpurified)

      Lane 1 : Mouse brain lysate
      Lane 2 : Rat brain lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 43 kDa



      Blocking and diluting buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse colon tissue sections labeling NDRG1 with purified ab133572 at 1:2000 dilution (0.59 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). An ImmunoHistoProbe one step HRP Polymer (ready to use) was used as a secondary antibody at 1:0 dilution. PBS instead of the primary antibody was used as a negative control. Hematoxylin was used as a counterstain.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung adenocarcinoma tissue sections labeling NDRG1 with purified ab133572 at 1:2000 dilution (0.59 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). An ImmunoHistoProbe one step HRP Polymer (ready to use) was used as a secondary antibody at 1:0 dilution. PBS instead of the primary antibody was used as a negative control. Hematoxylin was used as a counterstain.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling NDRG1 with purified ab133572 at 1:2000 dilution (0.59 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). An ImmunoHistoProbe one step HRP Polymer (ready to use) was used as a secondary antibody at 1:0 dilution. PBS instead of the primary antibody was used as a negative control. Hematoxylin was used as a counterstain.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat kidney tissue sections labeling NDRG1 with purified ab133572 at 1:2000 dilution (0.59 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). An ImmunoHistoProbe one step HRP Polymer (ready to use) was used as a secondary antibody at 1:0 dilution. PBS instead of the primary antibody was used as a negative control. Hematoxylin was used as a counterstain.

    • Lane 1 (input): HeLa(Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
      Lane 2 (+): ab133572 & HeLa whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab133572 in HeLa whole cell lysate

      Ab133572 (Purified) at 1:500 dilution (2.344 µg/ml) immunoprecipitating NDRG1 in HeLa whole cell lysate. For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used as the secondary antibody at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM /TBST .

    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon tissue labelling NDRG1 using ab133572 at a 1/250 dilution.
    • All lanes : Anti-NDRG1 antibody [EPR5592] (ab133572) at 1/10000 dilution

      Lane 1 : Fetal kidney lysate
      Lane 2 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 43 kDa
      Observed band size: 48 kDa
      why is the actual band size different from the predicted?

    References

    This product has been referenced in:
    • Liu W  et al. A new facet of NDRG1 in pancreatic ductal adenocarcinoma: Suppression of glycolytic metabolism. Int J Oncol 50:1792-1800 (2017). Read more (PubMed: 28350132) »
    • Gerdes MJ  et al. Highly multiplexed single-cell analysis of formalin-fixed, paraffin-embedded cancer tissue. Proc Natl Acad Sci U S A 110:11982-7 (2013). ICC/IF ; Human . Read more (PubMed: 23818604) »
    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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