Anti-NFAM1 antibody (ab203206)
Key features and details
- Rabbit polyclonal to NFAM1
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NFAM1 antibody -
Description
Rabbit polyclonal to NFAM1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human NFAM1 aa 100-200 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q8NET5 -
Positive control
- Human lung carcinoma tissue; Mouse splenocytes
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab203206 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
1/20 - 1/100.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at 1/50 - 1/200 with fluorescent detection methods. |
Notes |
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Flow Cyt
1/20 - 1/100. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use at 1/50 - 1/200 with fluorescent detection methods. |
Target
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Relevance
NFAM1 activates cytokine gene promoters such as the IL13 and TNF-alpha promoters. It contains an immunoreceptor tyrosine-based activation motif (ITAM) and is thought to regulate the signaling and development of B-cells. -
Cellular localization
Type I membrane protein. -
Database links
- Entrez Gene: 150372 Human
- Entrez Gene: 74039 Mouse
- Omim: 608740 Human
- SwissProt: Q8NET5 Human
- SwissProt: Q8R4V1 Mouse
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Alternative names
- Calcinerin/NFAT activating ITAM containing protein antibody
- CNAIP antibody
- NFAT activation molecule 1 precursor antibody
Images
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Immunohistochemical analysis of formalin-fixed and paraffin embedded Human lung carcinoma tissue labeling NFAM1 using ab203206 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
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Flow cytometry analysis of Mouse splenocytes labeling NFAM1 using ab203206 at 1/100 dilution for 40 minutes (green) followed by a FITC conjugated secondary compared to control cells (blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab203206 has not yet been referenced specifically in any publications.