Anti-NIPSNAP1 antibody (ab67324)
Key features and details
- Rabbit polyclonal to NIPSNAP1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NIPSNAP1 antibody
See all NIPSNAP1 primary antibodies -
Description
Rabbit polyclonal to NIPSNAP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- human brain tissue lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab67324 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
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ICC/IF |
Use a concentration of 20 µg/ml.
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IHC-P |
Use a concentration of 2.5 µg/ml.
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Notes |
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WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa). |
ICC/IF
Use a concentration of 20 µg/ml. |
IHC-P
Use a concentration of 2.5 µg/ml. |
Target
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Relevance
NIPSNAP is a member of an evolutionarily well conserved gene family and has a strong sequence similarity to the central portion of a protein encoded by C. elegans chromosome III between a 4-nitrophenylphosphatase (NIP) domain and non-neuronal SNAP25-like protein. Recent studies have indicated that NIPSNAP is involved in the regulation of the Ca2+-selective transient receptor potential vanilloid channel 6 (TRPV6). NIPSNAP1 associates with TRPV6 at the plasma membrane and inhibits TRPV6 currents. Other studies show that NIPSNAP’s expression is reduced in the phenylketonuria (PKU) mouse brain, suggesting that NIPSNAP may play a role in memory. -
Cellular localization
Mitochondrial -
Database links
- Entrez Gene: 8508 Human
- Omim: 603249 Human
- SwissProt: Q9BPW8 Human
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Alternative names
- NIPSNAP 1 antibody
- Nipsnap homolog 1 (C. elegans) antibody
- Protein NipSnap homolog 1 antibody
Images
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Immunofluorescence of NIPSNAP1 in Human brain tissue with ab67324 at 20 ug/mL.
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Lane 1 : Anti-NIPSNAP1 antibody (ab67324) at 0.5 µg/ml
Lane 2 : Anti-NIPSNAP1 antibody (ab67324) at 1 µg/ml
All lanes : human brain tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 33 kDa
Observed band size: 33 kDa -
ab67324 at 2.5 µg/ml staining NIPSNAP1 in human prostate tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab67324 has not yet been referenced specifically in any publications.