Anti-NKG2D antibody (ab203353)
Key features and details
- Rabbit polyclonal to NKG2D
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NKG2D antibody
See all NKG2D primary antibodies -
Description
Rabbit polyclonal to NKG2D -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Rat NKG2D aa 100-200 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O70215 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab203353 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
1/20 - 1/100.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
(or 1/50 -1/200 using a fluorescent secondary antibody). |
Notes |
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Flow Cyt
1/20 - 1/100. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. (or 1/50 -1/200 using a fluorescent secondary antibody). |
Target
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Function
Receptor for MICA, MICB, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4. Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. Involved in the immune surveillance exerted by T- and B-lymphocytes. -
Tissue specificity
Natural killer cells. Expressed on essentially all CD56+CD3- NK cells from freshly isolated PBMC. Also detected in gamma-delta cells and CD8+ alpha-beta T-cells. Expressed in interferon-producing killer dendritic cells (IKDCs). -
Sequence similarities
Contains 1 C-type lectin domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 100528032 Human
- Entrez Gene: 22914 Human
- Entrez Gene: 27007 Mouse
- Omim: 611817 Human
- SwissProt: P26718 Human
- SwissProt: O54709 Mouse
- Unigene: 387787 Human
- Unigene: 8217 Mouse
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Alternative names
- CD314 antibody
- CD314 antigen antibody
- D12S2489E antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed, paraffin embedded Human nasopharyngeal carcinoma tissue labeling NKG2D with ab203353 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
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Mouse thymus cells probed with ab203353 (green) at 1:100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)
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ab203353 staining NKG2D in mouse lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 20% serum for 20 minutes at 37°C; antigen retrieval was by heat mediation in a citrate buffer for 15 minutes. Samples were incubated with primary antibody (1/400 in blocking buffer) overnight at 4°C, followed by a conjugated secondary and DAB staining.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab203353 has been referenced in 9 publications.
- Wang H et al. CDX2 enhances natural killer cell-mediated immunotherapy against head and neck squamous cell carcinoma through up-regulating CXCL14. J Cell Mol Med 25:4596-4607 (2021). PubMed: 33733587
- Lee GH et al. Clinical Impact of Natural Killer Group 2D Receptor Expression and That of Its Ligand in Ovarian Carcinomas: A Retrospective Study. Yonsei Med J 62:288-297 (2021). PubMed: 33779082
- Čepcová D et al. The protective effect of 1-methyltryptophan isomers in renal ischemia-reperfusion injury is not exclusively dependent on indolamine 2,3-dioxygenase inhibition. Biomed Pharmacother 135:111180 (2021). PubMed: 33433354
- Spielmann J et al. Effects of obesity on NK cells in a mouse model of postmenopausal breast cancer. Sci Rep 10:20606 (2020). PubMed: 33244094
- Kravtsova-Ivantsiv Y et al. Excess of the NF-?B p50 subunit generated by the ubiquitin ligase KPC1 suppresses tumors via PD-L1- and chemokines-mediated mechanisms. Proc Natl Acad Sci U S A 117:29823-29831 (2020). PubMed: 33168738
- Mackiewicz Z et al. Sjögren's Syndrome: Concerted Triggering of Sicca Conditions. J Immunol Res 2019:2075803 (2019). PubMed: 30723748
- Zhang K et al. Splenic NKG2D confers resilience versus susceptibility in mice after chronic social defeat stress: beneficial effects of (R)-ketamine. Eur Arch Psychiatry Clin Neurosci N/A:N/A (2019). PubMed: 31875248
- Mo Z et al. Ultrasound-guided radiofrequency ablation enhances natural killer-mediated antitumor immunity against liver cancer. Oncol Lett 15:7014-7020 (2018). PubMed: 29725428
- Yin X et al. Role of NKG2D in cytokine-induced killer cells against lung cancer. Oncol Lett 13:3139-3143 (2017). PubMed: 28529563