Product nameAnti-NLK antibody
See all NLK primary antibodies
DescriptionRabbit polyclonal to NLK
Tested applicationsSuitable for: IP, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Recombinant fragment containing a sequence corresponding to a region within amino acids 284-500 of Human NLK (AAH64663)
- HeLa, A431 and H1299 cells
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 1.21% Tris, 0.75% Glycine
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab97642 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||1/500 - 1/3000. Predicted molecular weight: 58 kDa.|
|ICC/IF||1/100 - 1/200.|
FunctionRole in cell fate determination, required for differentiation of bone marrow stromal cells. Acts downstream of MAP3K7 and HIPK2 to negatively regulate the canonical Wnt/beta-catenin signaling pathway and the phosphorylation and destruction of the MYB transcription factor. May suppress a wide range of transcription factors by phosphorylation of the coactivator, CREBBP (By similarity). Involved in TGFbeta-mediated mesoderm induction, acting downstream of MAP3K7/TAK1 to phosphorylate STAT3.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
modificationsDually phosphorylated on Thr-303 and Tyr-305, which activates the enzyme.
Cellular localizationNucleus. Cytoplasm. Predominantly nuclear. A smaller fraction is cytoplasmic.
- Information by UniProt
- DKFZp761G1211 antibody
- FLJ21033 antibody
- LAK1 antibody
This product has been referenced in:
- Li CZ et al. RIP1 regulates TNF-a-mediated lymphangiogenesis and lymphatic metastasis in gallbladder cancer by modulating the NF-?B-VEGF-C pathway. Onco Targets Ther 11:2875-2890 (2018). Read more (PubMed: 29844685) »
- Huang T et al. A regulatory circuit of miR-125b/miR-20b and Wnt signalling controls glioblastoma phenotypes through FZD6-modulated pathways. Nat Commun 7:12885 (2016). Read more (PubMed: 27698350) »