Overview

  • Product name
    Anti-NMDAR2B (phospho S1303) antibody [EP1858Y]
    See all NMDAR2B primary antibodies
  • Description
    Rabbit monoclonal [EP1858Y] to NMDAR2B (phospho S1303)
  • Host species
    Rabbit
  • Specificity
    ab81271 detects NMDAR2B when phosphorylated at serine 1303
  • Tested applications
    Suitable for: Dot blot, WB, ICCmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding serine 1303 of human NMDAR2B.

  • Positive control
    • WB: Human, mouse and rat brain tissue lysates.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab81271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot 1/1000.
WB 1/1000 - 1/5000. Predicted molecular weight: 166 kDa.
ICC 1/100.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.
    • Tissue specificity
      Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia.
    • Sequence similarities
      Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily.
    • Cellular localization
      Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • AW490526 antibody
      • EIEE27 antibody
      • Glutamate [NMDA] receptor subunit epsilon 2 antibody
      • Glutamate [NMDA] receptor subunit epsilon-2 antibody
      • Glutamate Receptor Ionotropic N Methyl D Aspartate 2B antibody
      • Glutamate Receptor Ionotropic N Methyl D Aspartate subunit 2B antibody
      • Glutamate receptor ionotropic NMDA2B antibody
      • Glutamate receptor subunit epsilon 2 antibody
      • Glutamate receptor, ionotropic, NMDA2B (epsilon 2) antibody
      • GRIN 2B antibody
      • GRIN2B antibody
      • hNR 3 antibody
      • hNR3 antibody
      • MGC142178 antibody
      • MGC142180 antibody
      • MRD6 antibody
      • N methyl D asparate receptor channel subunit epsilon 2 antibody
      • N methyl D aspartate receptor subtype 2B antibody
      • N methyl D aspartate receptor subunit 2B antibody
      • N methyl D aspartate receptor subunit 3 antibody
      • N-methyl D-aspartate receptor subtype 2B antibody
      • N-methyl-D-aspartate receptor subunit 3 antibody
      • NMDA NR2B antibody
      • NMDA R2B antibody
      • NMDAR2B antibody
      • NMDE2 antibody
      • NMDE2_HUMAN antibody
      • NME2 antibody
      • NR2B antibody
      • NR3 antibody
      see all

    Images

    • All lanes : Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271) at 1/5000 dilution

      Lane 1 : Rat brain tissue lysate
      Lane 2 : Rat brain tissue lysate. The membrane was incubated with alkaline phosphatase.

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 166 kDa
      Observed band size: 180 kDa
      why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271) at 1/5000 dilution

      Lane 1 : Human brain tissue lysate
      Lane 2 : Human brain tissue lysate. The membrane was incubated with alkaline phosphatase.

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 166 kDa
      Observed band size: 180 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST.

    • Dot blot analysis of NMDAR2B (phospho S1303) phospho peptide (Lane 1) and NMDAR2B non-phospho peptide (Lane 2) labelling NMDAR2B (phospho S1303) with ab81271 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000 dilution. Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST.

    • All lanes : Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271) at 1/5000 dilution

      Lane 1 : Mouse brain lysates (untreated)
      Lane 2 : Mouse brain lysates (treated with AP)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP labelled IgG at 1/2000 dilution

      Predicted band size: 166 kDa
      Observed band size: 180 kDa why is the actual band size different from the predicted?

    References

    This product has been referenced in:
    • Li N  et al. LPM580098, a Novel Triple Reuptake Inhibitor of Serotonin, Noradrenaline, and Dopamine, Attenuates Neuropathic Pain. Front Pharmacol 10:53 (2019). Read more (PubMed: 30837867) »
    • Wang XS  et al. Anxiolytic effects of Formononetin in an inflammatory pain mouse model. Mol Brain 12:36 (2019). Read more (PubMed: 30961625) »
    See all 15 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (brain)
    Gel Running Conditions
    Non-reduced Denaturing (15% SDS-PAGE)
    Loading amount
    8 µg
    Treatment
    RIPA & 1%Protease inhibitor for 15min on ice
    Specification
    brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Nov 08 2018

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