Anti-NMRAL1 antibody (ab272637)
Key features and details
- Rabbit polyclonal to NMRAL1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NMRAL1 antibody -
Description
Rabbit polyclonal to NMRAL1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human NMRAL1 aa 1-150.
Database link: Q9HBL8 -
Positive control
- WB: HEK-293T lysate. IHC-P: Human rectum tissue. ICC/IF: MCF7.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272637 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Redox sensor protein. Undergoes restructuring and subcellular redistribution in response to changes in intracellular NADPH/NADP(+) levels. At low NADPH concentrations the protein is found mainly as a monomer, and binds argininosuccinate synthase (ASS1), the enzyme involved in nitric oxide synthesis. Association with ASS1 impairs its activity and reduces the production of nitric oxide, which subsecuently prevents apoptosis. Under normal NADPH concentrations, the protein is found as a dimer and hides the binding site for ASS1. The homodimer binds one molecule of NADPH. Has higher affinity for NADPH than for NADP(+). Binding to NADPH is necessary to form a stable dimer. -
Sequence similarities
Belongs to the NmrA-type oxidoreductase family. -
Cellular localization
Cytoplasm. Cytoplasm > perinuclear region. Nucleus. Under normal redox growth conditions localizes in the cytoplasm and perinuclear region. Nuclear localization is promoted by increased intracellular nitric oxide and reduced NADPH/NADP(+) ratios. - Information by UniProt
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Database links
- Entrez Gene: 57407 Human
- SwissProt: Q9HBL8 Human
- Unigene: 288969 Human
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Alternative names
- FLJ25918 antibody
- HSCARG antibody
- NmrA like family domain containing 1 antibody
see all
Images
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All lanes : Anti-NMRAL1 antibody (ab272637) at 0.4 µg/ml
Lane 1 : Control (vector only transfected HEK-293T lysate)
Lane 2 : NMRAL1 over-expression lysate (mammalian HEK-293T cells) -
Paraffin-embedded formalin-fixed Human rectum tissue stained for NMRAL1 using ab272637 at 1/200 in immunohistochemical analysis.
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PFA fixed, Triton X-100 permeablized human cell line MCF7 labeling NMRAL1 (green) using ab272637 at 2 ug/ml in ICC/IF analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab272637 has not yet been referenced specifically in any publications.