Anti-NOL10 antibody (ab235529)
Key features and details
- Rabbit polyclonal to NOL10
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NOL10 antibody
See all NOL10 primary antibodies -
Description
Rabbit polyclonal to NOL10 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human NOL10 aa 1-210.
Database link: Q9BSC4 -
Positive control
- WB: K562 whole cell lysate. IHC-P: Human pancreas and small intestine tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab235529 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/20 - 1/200.
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WB |
1/500 - 1/2000. Predicted molecular weight: 80 kDa.
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Notes |
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IHC-P
1/20 - 1/200. |
WB
1/500 - 1/2000. Predicted molecular weight: 80 kDa. |
Target
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Sequence similarities
Belongs to the WD repeat NOL10/ENP2 family.
Contains 5 WD repeats. -
Cellular localization
Nucleus > nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 79954 Human
- SwissProt: Q9BSC4 Human
- Unigene: 222494 Human
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Alternative names
- FLJ13938 antibody
- FLJ14075 antibody
- H_NH0074G24.1 antibody
see all
Images
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Anti-NOL10 antibody (ab235529) at 1/500 dilution + K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Secondary
Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 80 kDa -
Paraffin-embedded human pancreas tissue stained for NOL10 using ab235529 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human small intestine tissue stained for NOL10 using ab235529 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab235529 has not yet been referenced specifically in any publications.