Anti-NOX1 antibody (ab55831)
Key features and details
- Rabbit polyclonal to NOX1
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NOX1 antibody
See all NOX1 primary antibodies -
Description
Rabbit polyclonal to NOX1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to C terminal residues of human NOX1.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified by antigen specific affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab55831 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (2) |
Use a concentration of 1 µg/ml.
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Notes |
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ICC/IF
Use a concentration of 1 µg/ml. |
Target
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Function
NOH-1S is a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes and other tissues. It participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H(+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain. -
Tissue specificity
NOH-1L is detected in colon, uterus, prostate, and colon carcinoma, but not in peripheral blood leukocytes. NOH-1S is detected only in colon and colon carcinoma cells. -
Sequence similarities
Contains 1 FAD-binding FR-type domain.
Contains 1 ferric oxidoreductase domain. -
Cellular localization
Cell projection > invadopodium membrane. - Information by UniProt
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Database links
- Entrez Gene: 27035 Human
- Omim: 300225 Human
- SwissProt: Q9Y5S8 Human
- Unigene: 592227 Human
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Alternative names
- GP91 2 antibody
- Mitogenic oxidase (pyridine nucleotide dependent superoxide generating) antibody
- Mitogenic oxidase 1 antibody
see all
Images
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ICC/IF image of ab55831 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55831, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (45)
ab55831 has been referenced in 45 publications.
- Cremonini E et al. Supplementation with cyanidin and delphinidin mitigates high fat diet-induced endotoxemia and associated liver inflammation in mice. Food Funct 13:781-794 (2022). PubMed: 34981106
- Deng M et al. Scutellarin acts on the AR-NOX axis to remediate oxidative stress injury in a mouse model of cerebral ischemia/reperfusion injury. Phytomedicine 103:154214 (2022). PubMed: 35689902
- Zhang W et al. GPR43 regulation of mitochondrial damage to alleviate inflammatory reaction in sepsis. Aging (Albany NY) 13:22588-22610 (2021). PubMed: 34584017
- Del Gaudio I et al. Neonatal HDL Counteracts Placental Vascular Inflammation via S1P-S1PR1 Axis. Int J Mol Sci 21:N/A (2020). PubMed: 31991780
- Jiao P et al. The protective effect of doxofylline against lipopolysaccharides (LPS)-induced activation of NLRP3 inflammasome is mediated by SIRT1 in human pulmonary bronchial epithelial cells. Artif Cells Nanomed Biotechnol 48:687-694 (2020). PubMed: 32098511