Anti-NOX2/gp91phox antibody [54.1] (ab80897)
Key features and details
- Mouse monoclonal [54.1] to NOX2/gp91phox
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-NOX2/gp91phox antibody [54.1]
See all NOX2/gp91phox primary antibodies -
Description
Mouse monoclonal [54.1] to NOX2/gp91phox -
Host species
Mouse -
Specificity
This antibody is specific for NOX2/gp91phox 382-PKIAVDGP-389. -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
54.1 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab80897 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
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ICC/IF |
Use a concentration of 1 µg/ml.
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa). |
ICC/IF
Use a concentration of 1 µg/ml. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc. -
Involvement in disease
Defects in CYBB are a cause of chronic granulomatous disease X-linked (XCGD) [MIM:306400]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections. -
Sequence similarities
Contains 1 FAD-binding FR-type domain.
Contains 1 ferric oxidoreductase domain. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 1536 Human
- Omim: 300481 Human
- SwissProt: P04839 Human
- Unigene: 292356 Human
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Alternative names
- AMCBX2 antibody
- CGD antibody
- CGD91-phox antibody
see all
Images
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All lanes : Anti-NOX2/gp91phox antibody [54.1] (ab80897) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : Human lymph node tissue lysate - total protein (ab29871)
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 65 kDa
Observed band size: 65 kDa
Additional bands at: 29 kDa, 35 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes -
ICC/IF image of ab80897 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80897, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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IHC image of ab80897 staining in normal human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab80897, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (16)
ab80897 has been referenced in 16 publications.
- Jiang Z et al. NOX2 and NOX5 are increased in cardiac microvascular endothelium of deceased COVID-19 patients. Int J Cardiol 370:454-462 (2023). PubMed: 36332749
- Maneta E et al. G-CSF induces CD15+ CD14+ cells from granulocytes early in the physiological environment of pregnancy and the cancer immunosuppressive microenvironment. Clin Transl Immunology 11:e1395 (2022). PubMed: 35602884
- Chung EJ et al. Natural variation in macrophage polarization and function impact pneumocyte senescence and susceptibility to fibrosis. Aging (Albany NY) 14:7692-7717 (2022). PubMed: 36173617
- Jin K et al. NOTCH-induced rerouting of endosomal trafficking disables regulatory T cells in vasculitis. J Clin Invest 131:N/A (2021). PubMed: 32960812
- Hao Y et al. Transcriptomic Signatures and Functional Network Analysis of Chronic Rhinosinusitis With Nasal Polyps. Front Genet 12:609754 (2021). PubMed: 33603773