Anti-NPEPPS antibody (ab251735)
Key features and details
- Rabbit polyclonal to NPEPPS
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NPEPPS antibody
See all NPEPPS primary antibodies -
Description
Rabbit polyclonal to NPEPPS -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Recombinant fragment corresponding to Human NPEPPS aa 700-850.
Database link: P55786 -
Positive control
- IHC-P: Human duodenum, kidney, Fallopian tube and testis tissue. ICC/IF: A549 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab251735 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
Notes |
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IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
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Function
Aminopeptidase with broad substrate specificity for several peptides. Involved in proteolytic events essential for cell growth and viability. May act as regulator of neuropeptide activity. Plays a role in the antigen-processing pathway for MHC class I molecules. Involved in the N-terminal trimming of cytotoxic T-cell epitope precursors. Digests the poly-Q peptides found in many cellular proteins. Digests tau from normal brain more efficiently than tau from Alzheimer disease brain. -
Tissue specificity
Detected in liver, epithelium of renal tubules, epithelium of small and large intestine, gastric epithelial cells, and alveoli of the lung (at protein level). -
Sequence similarities
Belongs to the peptidase M1 family. -
Cellular localization
Cytoplasm > cytosol. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 9520 Human
- Entrez Gene: 19155 Mouse
- Omim: 606793 Human
- SwissProt: P55786 Human
- SwissProt: Q11011 Mouse
- Unigene: 443837 Human
- Unigene: 29824 Mouse
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Alternative names
- AAP-S antibody
- Aminopeptidase puromycin sensitive antibody
- Cytosol alanyl aminopeptidase antibody
see all
Images
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PFA-fixed, Triton X-100 permeabilized A549 (human lung carcinoma cell line) cells stained for NPEPPS (green) using ab251735 at 4 µg/ml in ICC/IF.
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Formalin-fixed, paraffin-embedded human duodenum tissue stained for NPEPPS with ab251735 at a 1/20 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human kidney tissue stained for NPEPPS with ab251735 at a 1/20 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human Fallopian tube tissue stained for NPEPPS with ab251735 at a 1/20 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human testis tissue stained for NPEPPS with ab251735 at a 1/20 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab251735 has not yet been referenced specifically in any publications.