Overview

  • Product name
    Anti-NPTN antibody
  • Description
    Rabbit polyclonal to NPTN
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 108-157 (SAPKANATIE VKAAPDITGH KRSENKNEGQ DATMYCKSVG YPHPDWIWRK) of Human NPTN (NP_059429).

  • Positive control
    • Fetal muscle lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab83063 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 32 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Probable homophilic and heterophilic cell adhesion molecule involved in long term potentiation at hippocampal excitatory synapses through activation of p38MAPK. May also regulate neurite outgrowth by activating the FGFR1 signaling pathway. May play a role in synaptic plasticity.
  • Tissue specificity
    Isoform 1 is ubiquitously expressed. Isoform 2 is expressed in brain cortex and cerebellum (at protein level).
  • Sequence similarities
    Contains 3 Ig-like (immunoglobulin-like) domains.
  • Domain
    Some isoforms lack the first Ig-like domain which may confer homophilic adhesion activity. However, they can bind and activate FGFR1.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localization
    Cell membrane. Enriched at post-synaptic density.
  • Information by UniProt
  • Database links
  • Alternative names
    • GP55 antibody
    • GP65 antibody
    • Neuroplastin antibody
    • np55 antibody
    • np65 antibody
    • Nptn antibody
    • NPTN_HUMAN antibody
    • SDFR1 antibody
    • SDR-1 antibody
    • SDR1 antibody
    • stromal cell derived factor receptor 1 antibody
    • Stromal cell-derived receptor 1 antibody
    see all

Images

  • Anti-NPTN antibody (ab83063) at 1 µg/ml (5% skim milk / PBS buffer) + Fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 32 kDa
    Observed band size: 31 kDa
    why is the actual band size different from the predicted?



    Gel concentration: 12%

References

ab83063 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

Thank you for contacting us about the issues you are having with the FGFR1 and NPTN antibodies. Can you please send me a few details of your samples and the protocols you use for harvesting the samples and for blotting? In particular, I am interested in what the samples are, how much is loaded per lane of the gel, the efficacy of transfer to the blots, the blocking solution, and the concentrations or dilutions of the antibodies. You mentioned in our conversation that the lanes containing NPTN-transfected samples showed at least three bands, one specific, two not. Can you please tell me the size of all the bands that were observed? If you have an image of a blot, please attach it to your reply, if it is convenient. For the FGFR1 antibody, you said no bands were apparent. Did you try optimizing the protocol, for instance by loading more sample or increasing the antibody concentration? Both antibodies are covered by our Abpromise guarantee, so if I am unable to make useful suggestions, we can look into replacing the antibodies, or crediting the cost through Cedarlane. I look forward to your reply.

Read More
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain Tissue)
Loading amount
40 µg
Specification
Brain Tissue
Gel Running Conditions
Non-reduced Denaturing (10% Bis-Tris)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted May 27 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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