Overview

  • Product name

  • Description

    Sheep polyclonal to NPY1R
  • Host species

    Sheep
  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide: K-Q-A-S-P-V-A-F-K-K-I-N-N-N conjugated to KLH, corresponding to amino acids 365-378 of Human NPY1R

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab35336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/110.
WB Use at an assay dependent concentration. Predicted molecular weight: 44 kDa.

Target

Images

  • Anti-NPY1R antibody (ab35336) + Human Brain Lysate

    Predicted band size: 44 kDa

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (rat skin (free floating 50 µm))
Specification
rat skin (free floating 50 µm)
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton X-100, 0.2%
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jun 25 2012

Answer

Thank you for your phone call.
I am sorry to hear that the antibody is not working in frozen sections, but unfortunately we have not tried this application and thus do not know for sure if the antibody would work or not in IHC-Fr.

In order to help resolve this case, I'm happy to send youa testing discount code for having tried the antibody in an untested application.
In addition, I might suggest you to try including an enzymatic antigen retrieval step as this might helpwith obtaining a signal.


DISCOUNT CODE: xxx
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab35336 in IHC-Fr. This code will give you: 1 free PRIMARY ANTIBODYbefore the expiration date. To redeem this offer, please submit an Abreview for IHC-Fr and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

Read More
Application
Western blot
Sample
Mouse Tissue lysate - whole (brain cortex)
Loading amount
100 µg
Specification
brain cortex
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 8%

Dr. paolo mele

Verified customer

Submitted Feb 13 2007

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