Product nameNative human IgE protein (Azide free)
See all IgE proteins and peptides
Protein lengthFull length protein
Amino Acid Sequence 1
Amino Acid Sequence 2
Additional sequence informationObserved band of ~185kDa on SDS-PAGE
Our Abpromise guarantee covers the use of ab65866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC - Wholemount
Endotoxin level< 25.000 Eu/mg
Purity> 90 % SDS-PAGE.
Human IgE full length protein was purified by Protein L chromatography. As the IgE comes from a monoclonal cell line, there is no contamination of antibodies of other isotypes. The remaining contaminants are mainly components from Foetal Bovine Serum.
This product is purified Human IgE with kappa light chains produced in vitro from a monoclonal hybridoma.
This antigen is quite unique, because it is the only available IgE product, which originates from a human monoclonal cell line. Our IgE product shows superior advantage when compared to the traditionally purified polyclonal IgE from myeloma patients. A comparison which is highly superior on the following key parameters:.
- Less batch-to-batch variation
- Higher degree of purity (>90%)
- A more uniform product (monoclonal and not polyclonal)
IgE has been used in a variety of cell stimulation assays, including the following cell types:
- Human airway smooth muscle (HASM) cells
- mast cells (MC)
- Human bronchial/tracheal smooth muscle (B/TSM) cells
- acute myelogenous leukemia (AML) blasts
- Human neutrophils
Low endotoxin levels below < 25 EU/mg allow use of IgE protein in cellular immunology and in cell proliferation studies.
1 mg of ab65866 is 380,000± 50,000 IU/ml.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Constituents: 0.00017% PBS, 0.00087% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Immunoglobulin heavy constant epsilon
RelevanceIgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.
Cellular localizationCell Membrane
This product has been referenced in:
- Vidal M et al. Development of quantitative suspension array assays for six immunoglobulin isotypes and subclasses to multiple Plasmodium falciparum antigens. J Immunol Methods N/A:N/A (2018). Read more (PubMed: 29397157) »
- Wan Z et al. Photocleavage-based affinity purification of biomarkers from serum: Application to multiplex allergy testing. PLoS One 13:e0191987 (2018). Read more (PubMed: 29389948) »