Native Staphylococcus aureus Protein A (Alkaline Phosphatase) (ab7345)
Key features and details
- Expression system: Native
- Purity: > 95% Protein A purified
- Suitable for: WB, Dot blot, ELISA, IHC-P, IHC-Fr
Description
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Product name
Native Staphylococcus aureus Protein A (Alkaline Phosphatase)
See all Protein A proteins and peptides -
Purity
> 95 % Protein A purified. -
Expression system
Native -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Native -
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Species
Staphylococcus aureus -
Predicted molecular weight
56 kDa
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Conjugation
Alkaline Phosphatase
Specifications
Our Abpromise guarantee covers the use of ab7345 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Dot blot
ELISA
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
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Form
Liquid -
Additional notes
Protein A Concentration: 1.0 mg/ml (by UV absorbance at 280 nm) This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using pNPP (p-nitrophenyl phosphate) as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,000 of the stock concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher. -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C. Do Not Freeze.
Preservative: 0.1% Sodium azide
Constituents: 0.00136% Zinc chloride, 0.0095% Magnesium chloride, 0.79% Tris HCl, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 1.5% BSA
BSA is IgG and Protease free
General Info
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Alternative names
- gG-binding protein A
- Immunoglobulin G binding protein A
- SPA
- Staphylococcal protein A
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Relevance
Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology. -
Cellular localization
Secreted; cell wall.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab7345 has not yet been referenced specifically in any publications.