Native human alpha 1 Antitrypsin protein (Active) (ab91136)
Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
Achieve higher consistency and quality standards with a premium grade bioactive protein
- High batch-to-batch consistency
- Optimal bioactivity
- Guaranteed identical to human native proteins
- >95% purity
- Ultra-low endotoxin levels: <0.005 Eu/µg
- Carrier and tag free
Description
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Product name
Native human alpha 1 Antitrypsin protein (Active)
See all alpha 1 Antitrypsin proteins and peptides -
Biological activity
When tested with active-site titrated porcine pancreatic trypsin using Na-Benzoyl-L-Arginine-para-Nitroanilide Hydrochloride (L-BAPNA) as substrate, it is 75-100% inhibitory.
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Purity
> 95 % SDS-PAGE. -
Expression system
Native -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Native -
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Species
Human -
Predicted molecular weight
52 kDa
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab91136 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Functional Studies
SDS-PAGE
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Form
Lyophilized -
Additional notes
Protein Determination: Extinction Coefficient (E) 0.1% at 280nm, 1cm pathway = 0.433
Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -80°C.
pH: 6.50
Constituents: 0.492% Sodium phosphate, 1.74% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with distilled water. Once reconstituted, ab91136 is stable for one week at 4°C.
General Info
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Alternative names
- A1A
- A1AT
- A1AT_HUMAN
see all -
Function
Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE). -
Tissue specificity
Ubiquitous. Expressed in leukocytes and plasma. -
Involvement in disease
Alpha-1-antitrypsin deficiency -
Sequence similarities
Belongs to the serpin family. -
Domain
The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable. -
Post-translational
modificationsN-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418. -
Cellular localization
Secreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum and Secreted, extracellular space, extracellular matrix. - Information by UniProt
Images
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SDS-PAGE: 4-12% Bis-Tris NuPAGE gel
Lane 1. 5 μg ab91136 (reduced/heated)
Lane 2. 10 μg ab91136 (reduced/heated)
Lane 3. 20 μg ab91136 (reduced/heated)
Lane 4. Molecular weight markers
Lane 5. 5 μg ab91136 (non-reduced/no heat)
Lane 6. 10 μg ab91136 (non-reduced/no heat)
Lane 7. 20 μg ab91136 (non-reduced/no heat) -
Anti-alpha 1 Antitrypsin antibody [B9] (ab9399) at 1 µg/ml +
Native human alpha 1 Antitrypsin protein (Active) (ab91136) at 0.1 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 30 seconds
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab91136 has been referenced in 2 publications.
- Esteghamat F et al. CELA2A mutations predispose to early-onset atherosclerosis and metabolic syndrome and affect plasma insulin and platelet activation. Nat Genet 51:1233-1243 (2019). PubMed: 31358993
- Muley MM et al. Prophylactic inhibition of neutrophil elastase prevents the development of chronic neuropathic pain in osteoarthritic mice. J Neuroinflammation 14:168 (2017). PubMed: 28835277