Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] (ab80299)
Key features and details
- Rabbit monoclonal [EPTPG1] to Nephrin (phospho Y1176 + Y1193)
- Suitable for: WB, Dot blot
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1]
See all Nephrin primary antibodies -
Description
Rabbit monoclonal [EPTPG1] to Nephrin (phospho Y1176 + Y1193) -
Host species
Rabbit -
Specificity
This antibody detects Nephrin when either tyrosines 1176 or 1193 are phosphorylated, but does not detect non-phosphorylated Nephrin. -
Tested applications
Suitable for: WB, Dot blotmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide within Mouse Nephrin (phospho Y1176 + Y1193). The exact sequence is proprietary.
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Positive control
- Transfected HEK293T cells
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPTPG1 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab80299 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Detects a band of approximately 138, 180 kDa (predicted molecular weight: 138 kDa).
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Dot blot |
Use at an assay dependent concentration.
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Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 138, 180 kDa (predicted molecular weight: 138 kDa). |
Dot blot
Use at an assay dependent concentration. |
Target
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Function
Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion. -
Tissue specificity
Specifically expressed in podocytes of kidney glomeruli. -
Involvement in disease
Defects in NPHS1 are the cause of nephrotic syndrome type 1 (NPHS1) [MIM:256300]; also known as Finnish congenital nephrosis (CNF). A renal disease characterized clinically by proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. Some affected individuals have an inherited steroid-resistant form and progress to end-stage renal failure. -
Sequence similarities
Belongs to the immunoglobulin superfamily.
Contains 1 fibronectin type-III domain.
Contains 8 Ig-like C2-type (immunoglobulin-like) domains. -
Developmental stage
In 23-week-old embryo found in epithelial podocytes of the periphery of mature and developing glomeruli. -
Post-translational
modificationsPhosphorylated on tyrosine residues. -
Cellular localization
Cell membrane. Predominantly located at podocyte slit diaphragm between podocyte foot processes. Also associated with podocyte apical plasma membrane. - Information by UniProt
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Database links
- Entrez Gene: 4868 Human
- Entrez Gene: 54631 Mouse
- Omim: 602716 Human
- SwissProt: O60500 Human
- SwissProt: Q9QZS7 Mouse
- Unigene: 122186 Human
- Unigene: 437830 Mouse
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Alternative names
- CNF antibody
- Nephrin antibody
- Nephrosis 1 congenital Finnish type antibody
see all
Images
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All lanes : Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] (ab80299) at 1/1000 dilution
Lane 1 : HEK293T (human embryonic kidney epithelial cell) transfected with myc-tagged human Nephrin expression vector, whole cell lysate
Lane 2 : HEK293T (human embryonic kidney epithelial cell) transfected with myc-tagged human Nephrin and myc-tagged Src expression vector, whole cell lysate
Lane 3 : HEK293T (human embryonic kidney epithelial cell) transfected with myc-tagged human Nephrin and myc-tagged Src expression vector, whole cell lysate. Then the membrane was incubated with phosphatase.
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 138 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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Dot blot analysis of Nephrin (pY1176 + pY1193) phospho peptide (Lane 1), Nephrin (pY1176) phospho peptide (Lane 2), Nephrin (pY1193) phospho peptide (Lane 3) and Nephrin non-phospho peptide (Lane 4) labelling Nephrin (pY1176 + pY1193) with ab80299 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000. Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST.
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All lanes : Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] (ab80299) at 1/50000 dilution
Lane 1 : HEK293T (human embryonic kidney epithelial cell) transfected with wild type CD 16/7 Nephrin expression vector, whole cell lysate
Lane 2 : HEK293T (human embryonic kidney epithelial cell) transfected with wild type CD 16/7 Nephrin and active tyrosine kinase (v-Src) expression vector, whole cell lysate
Lane 3 : HEK293T (human embryonic kidney epithelial cell) transfected with Y1176F mutant CD 16/7 Nephrin and active tyrosine kinase (v-Src) expression vector, whole cell lysate
Lane 4 : HEK293T (human embryonic kidney epithelial cell) transfected with Y1193F mutant CD 16/7 Nephrin and active tyrosine kinase (v-Src) expression vector, whole cell lysate
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 138 kDa
Observed band size: 138,180 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab80299 has been referenced in 9 publications.
- Jansen J et al. Human pluripotent stem cell-derived kidney organoids for personalized congenital and idiopathic nephrotic syndrome modeling. Development 149:N/A (2022). PubMed: 35417019
- Cara-Fuentes G et al. Pulmonary surfactants and the respiratory-renal connection in steroid-sensitive nephrotic syndrome of childhood. iScience 25:104694 (2022). PubMed: 35847557
- Solanki AK et al. Phosphorylation of slit diaphragm proteins NEPHRIN and NEPH1 upon binding of HGF promotes podocyte repair. J Biol Chem 297:101079 (2021). PubMed: 34391780
- Martin CE et al. Multivalent nephrin-Nck interactions define a threshold for clustering and tyrosine-dependent nephrin endocytosis. J Cell Sci 133:N/A (2020). PubMed: 31974115
- Chun J et al. Recruitment of APOL1 kidney disease risk variants to lipid droplets attenuates cell toxicity. Proc Natl Acad Sci U S A 116:3712-3721 (2019). PubMed: 30733285
- Wei C et al. SHROOM3-FYN Interaction Regulates Nephrin Phosphorylation and Affects Albuminuria in Allografts. J Am Soc Nephrol 29:2641-2657 (2018). PubMed: 30341149
- Lin JS et al. ARF6 mediates nephrin tyrosine phosphorylation-induced podocyte cellular dynamics. PLoS One 12:e0184575 (2017). PubMed: 28880939
- Kapodistria K et al. Nephrin, a transmembrane protein, is involved in pancreatic beta-cell survival signaling. Mol Cell Endocrinol 400:112-28 (2015). PubMed: 25448064
- Jeon J et al. Dynamin-mediated Nephrin Phosphorylation Regulates Glucose-stimulated Insulin Release in Pancreatic Beta Cells. J Biol Chem 287:28932-42 (2012). WB ; Human . PubMed: 22718751