Recombinant Anti-Nephrin (phospho Y1217) antibody [EPTPG3] (ab80298)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPTPG3] to Nephrin (phospho Y1217)
- Suitable for: WB, Dot blot
- Reacts with: Human
Overview
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Product name
Anti-Nephrin (phospho Y1217) antibody [EPTPG3]
See all Nephrin primary antibodies -
Description
Rabbit monoclonal [EPTPG3] to Nephrin (phospho Y1217) -
Host species
Rabbit -
Tested applications
Suitable for: WB, Dot blotmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide within Mouse Nephrin (phospho Y1217). The exact sequence is proprietary.
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Positive control
- WB: 293T transfected with myc-tagged human Nephrin and myc-tagged Src expression vectors.
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General notes
This product has switched from a hybridoma to recombinant production method on 20th November 2023.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPTPG3 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab80298 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000. Detects a band of approximately 138 kDa (predicted molecular weight: 138 kDa).
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Dot blot |
1/1000.
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Notes |
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WB
1/10000. Detects a band of approximately 138 kDa (predicted molecular weight: 138 kDa). |
Dot blot
1/1000. |
Target
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Function
Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion. -
Tissue specificity
Specifically expressed in podocytes of kidney glomeruli. -
Involvement in disease
Defects in NPHS1 are the cause of nephrotic syndrome type 1 (NPHS1) [MIM:256300]; also known as Finnish congenital nephrosis (CNF). A renal disease characterized clinically by proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. Some affected individuals have an inherited steroid-resistant form and progress to end-stage renal failure. -
Sequence similarities
Belongs to the immunoglobulin superfamily.
Contains 1 fibronectin type-III domain.
Contains 8 Ig-like C2-type (immunoglobulin-like) domains. -
Developmental stage
In 23-week-old embryo found in epithelial podocytes of the periphery of mature and developing glomeruli. -
Post-translational
modificationsPhosphorylated on tyrosine residues. -
Cellular localization
Cell membrane. Predominantly located at podocyte slit diaphragm between podocyte foot processes. Also associated with podocyte apical plasma membrane. - Information by UniProt
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Database links
- Entrez Gene: 4868 Human
- Entrez Gene: 54631 Mouse
- Omim: 602716 Human
- SwissProt: O60500 Human
- SwissProt: Q9QZS7 Mouse
- Unigene: 122186 Human
- Unigene: 437830 Mouse
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Alternative names
- CNF antibody
- Nephrin antibody
- Nephrosis 1 congenital Finnish type antibody
see all
Images
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All lanes : Anti-Nephrin (phospho Y1217) antibody [EPTPG3] (ab80298) at 1/10000 dilution
Lane 1 : 293T (Human embryonic kidney epithelial cell) transfected with a myc-tagged empty vector whole cell lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) transfected with a myc-tagged human Nephrin expression vector, whole cell lysate
Lane 3 : 293T (Human embryonic kidney epithelial cell) transfected with a myc-tagged Src expression vector, whole cell lysate
Lane 4 : 293T (Human embryonic kidney epithelial cell) transfected with myc-tagged human Nephrin and myc-tagged Src expression vectors, whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 138 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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All lanes : Anti-Nephrin (phospho Y1217) antibody [EPTPG3] (ab80298) at 1/10000 dilution
Lane 1 : 293T (Human embryonic kidney epithelial cell) transfected with a myc-tagged empty vector whole cell lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) transfected with with myc-tagged human Nephrin and myc-tagged Src expression vectors, whole cell lysate
Lane 3 : 293T (Human embryonic kidney epithelial cell) transfected with myc-tagged human Nephrin and myc-tagged Src expression vectors, whole cell lysate 15 µg. Then the membrane was incubated with Alkaline phosphatase
Lysates/proteins at 15 µg per lane.
Predicted band size: 138 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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Dot blot analysis of Nephrin (pY1217) peptide and Nephrin non-phospho peptide labelling PKC alpha Nephrin (pY1217) with ab80298 at a 1/1000 dilution. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a 1/20,000 dilution. Blocking and dilution buffer: 5% NFDM/TBST.
Lane 1: Nephrin (pY1217) phospho peptide
Lane 2: Nephrin non-phospho peptide
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab80298 has been referenced in 8 publications.
- Solanki AK et al. Phosphorylation of slit diaphragm proteins NEPHRIN and NEPH1 upon binding of HGF promotes podocyte repair. J Biol Chem 297:101079 (2021). PubMed: 34391780
- Tian Y et al. Nestin protects podocyte from injury in lupus nephritis by mitophagy and oxidative stress. Cell Death Dis 11:319 (2020). PubMed: 32371936
- Yuan Q et al. Role of pyruvate kinase M2-mediated metabolic reprogramming during podocyte differentiation. Cell Death Dis 11:355 (2020). PubMed: 32393782
- New LA et al. Nephrin Tyrosine Phosphorylation Is Required to Stabilize and Restore Podocyte Foot Process Architecture. J Am Soc Nephrol 27:2422-35 (2016). PubMed: 26802179
- Kumagai T et al. Protein tyrosine phosphatase 1B inhibition protects against podocyte injury and proteinuria. Am J Pathol 184:2211-24 (2014). PubMed: 24951831
- Nezvitsky L et al. Complement-mediated glomerular injury is reduced by inhibition of protein-tyrosine phosphatase 1B. Am J Physiol Renal Physiol 307:F634-47 (2014). PubMed: 25056348
- Jeon J et al. Dynamin-mediated Nephrin Phosphorylation Regulates Glucose-stimulated Insulin Release in Pancreatic Beta Cells. J Biol Chem 287:28932-42 (2012). WB ; Human . PubMed: 22718751
- Azhibekov TA et al. TM4SF10 and ADAP interaction in podocytes: role in Fyn activity and nephrin phosphorylation. Am J Physiol Cell Physiol 301:C1351-9 (2011). PubMed: 21881001