Overview

  • Product name
    Anti-Nestin antibody - Neural Stem Cell Marker
    See all Nestin primary antibodies
  • Description
    Rabbit polyclonal to Nestin - Neural Stem Cell Marker
  • Host species
    Rabbit
  • Specificity
    Detects a band at 200kDa representing Nestin in MEFs. Denaturing of WB protein lysate causes loss of Nestin immunoreactivity in WB.
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Dog, Human
    Predicted to work with: Rat, Rabbit, Hamster
  • Immunogen

    Synthetic peptide:

    ALETESQDSAEPSGSEE

    , corresponding to amino acids 1481-1497 of Human Nestin.

  • General notes
    ab7659 has been upgraded from Fast Track status based on data obtained in a new batch of this antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab7659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Detects a band of approximately 200 kDa (predicted molecular weight: 176 kDa). Do not denature WB protein lysate, denaturing results in loss of Nestin specific immunoreactivity (See Abreview).
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function
      Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
    • Tissue specificity
      CNS stem cells.
    • Sequence similarities
      Belongs to the intermediate filament family.
    • Developmental stage
      Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.
    • Post-translational
      modifications
      Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
    • Information by UniProt
    • Database links
    • Alternative names
      • ESTM 46 antibody
      • FLJ 21841 antibody
      • FLJ21841 antibody
      • Intermediate filament protein antibody
      • Nbla00170 antibody
      • nes antibody
      • NEST_HUMAN antibody
      • Nestin antibody
      see all

    Images

    • Anti-Nestin antibody - Neural Stem Cell Marker (ab7659) at 1/2000 dilution + MEF whole cell lysate at 30 µg

      Secondary
      IRDye 800
      conjugated Goat anti-Rabbit IgG [H&L] at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size: 176 kDa
      Observed band size: 200 kDa
      why is the actual band size different from the predicted?

    • All lanes : Anti-Nestin antibody - Neural Stem Cell Marker (ab7659) at 1 µg/ml

      Lane 1 : Mouse neural precursors whole cell lysate reduced but not denatured
      Lane 2 : Mouse neural precursors whole cell lysate reduced and denatured

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit IgG HRP at 1/20000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 176 kDa
      Observed band size: 230 kDa why is the actual band size different from the predicted?


      Exposure time: 10 minutes


      A slightly higher band obtained than expected, but as predicted in the datasheet. Denaturing of the WB lysate results in a loss of Nestin specific immunoreactivity. NB: Equal protein loading was verified.

    References

    This product has been referenced in:
    • Veron AD  et al. Isolation and characterization of olfactory ecto-mesenchymal stem cells from eight mammalian genera. BMC Vet Res 14:17 (2018). ICC/IF . Read more (PubMed: 29343270) »
    • Dorraji SE  et al. Mesenchymal stem cells and T cells in the formation of Tertiary Lymphoid Structures in Lupus Nephritis. Sci Rep 8:7861 (2018). Read more (PubMed: 29777158) »
    See all 6 Publications for this product

    Customer reviews and Q&As

    1-10 of 12 Abreviews or Q&A

    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (neural precourser cell derived from iPSC)
    Specification
    neural precourser cell derived from iPSC
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Apr 13 2016

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Monkey Cell (Olfactory Stem Cells)
    Permeabilization
    Yes - 0,1% Triton X100
    Specification
    Olfactory Stem Cells
    Blocking step
    BSA as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative
    Formaldehyde

    Antoine Veron

    Verified customer

    Submitted Sep 11 2015

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Sheep Cell (Olfactory Stem Cell)
    Permeabilization
    Yes - 0,1% Triton X100
    Specification
    Olfactory Stem Cell
    Blocking step
    BSA as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative
    Formaldehyde

    Antoine Veron

    Verified customer

    Submitted Sep 08 2015

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Horse Cell (Olfactory Stem cells)
    Permeabilization
    Yes - 0,1% Triton X100
    Specification
    Olfactory Stem cells
    Blocking step
    BSA as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative
    Paraformaldehyde

    Antoine Veron

    Verified customer

    Submitted Jul 16 2015

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rabbit Cell (Olfactory stem cells)
    Permeabilization
    Yes - 0,1% Triton 100X
    Specification
    Olfactory stem cells
    Blocking step
    BSA as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative
    Paraformaldehyde

    Antoine Veron

    Verified customer

    Submitted Jun 22 2015

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Dog Cell (Olfactory Stem Cells)
    Permeabilization
    Yes - 0,1% Triton X100
    Specification
    Olfactory Stem Cells
    Blocking step
    BSA as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative
    Paraformaldehyde

    Antoine Veron

    Verified customer

    Submitted Jun 22 2015

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Microcebus (Mouse Lemur) Cell (Olfactory Stem Cells)
    Permeabilization
    Yes - 0,1% Triton X100
    Specification
    Olfactory Stem Cells
    Blocking step
    BSA as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative
    Paraformaldehyde

    Antoine Veron

    Verified customer

    Submitted Jun 22 2015

    Answer

    Thank you for contacting Abcam regarding ab7659. These applications have been listed as unsuitable as we have obtained a range of feedback stating that the antibody is staining nonspecifically in these applications.  If you have not used this antibody, you can return it to us.  Please contact us for additional details. I hope this information is helpful.  Please do not hesitate to contact us if you have any additional questions.

    Read More
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Brain)
    Specification
    Brain
    Fixative
    Acetone
    Permeabilization
    Yes - Methanol
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 18°C

    Abcam user community

    Verified customer

    Submitted Dec 18 2007

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (neural precursor cells/neurospheres)
    Loading amount
    10 µg
    Specification
    neural precursor cells/neurospheres
    Blocking step
    Milk as blocking agent for 20 minute(s) · Concentration: 5%

    Dr. Randal Moldrich

    Verified customer

    Submitted May 18 2006

    1-10 of 12 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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