Recombinant
RabMAb

Recombinant Anti-NeuN antibody [EPR21902] - BSA and Azide free (ab239345)

Overview

  • Product name
    Anti-NeuN antibody [EPR21902] - BSA and Azide free
    See all NeuN primary antibodies
  • Description
    Rabbit monoclonal [EPR21902] to NeuN - BSA and Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human NeuN aa 1-100. The exact sequence is proprietary.
    Database link: A6NFN3

  • Positive control
    • IHC-P: Human retina tissue.
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).

    ab239345 is a PBS-only buffer format of ab236869. Please refer to ab236869 for recommended dilutions, protocols, and image data.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab239345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 46, 48 kDa (predicted molecular weight: 34 kDa).
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function
    RNA-binding protein that regulates alternative splicing events.
  • Sequence similarities
    Contains 1 RRM (RNA recognition motif) domain.
  • Cellular localization
    Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ56884 antibody
    • FLJ58356 antibody
    • Fox-1 homolog C antibody
    • fox1 homolog C antibody
    • Fox3 antibody
    • FOX3NeuN antibody
    • hexaribonucleotide binding protein 3 antibody
    • HRNBP3 antibody
    • NEUN antibody
    • neuronal nuclei antibody
    • Rbfox3 antibody
    • RFOX3_HUMAN antibody
    • RNA binding protein fox-1 homolog 3 antibody
    • RNA binding protein, fox 1 homolog (C. elegans) 3 antibody
    see all

Images

  • NeuN was immunoprecipitated from 0.35 mg human fetal brain lysate with ab236869 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab236869 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Human fetal brain lysate 10 µg (Input).
    Lane 2: ab236869 IP in human fetal brain lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab236869 in human fetal brain lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 8 seconds.

    The molecular weight observed is consistent with what has been described in the literature (PMID:1483388).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236869).

  • Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling NeuN with ab236869 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining in neurons of human cerebrum (PMID:8813082; PMID:1483388; PMID:26085943). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236869).

  • Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling NeuN with ab236869 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining in granule cells of human cerebellum (PMID:8813082; PMID:1483388). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236869).

  • Immunohistochemical analysis of paraffin-embedded human retina tissue labeling NeuN with ab236869 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining in ganglion cells of human retina (PMID:8813082; PMID:1483388). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab236869).

References

ab239345 has not yet been referenced specifically in any publications.

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