Recombinant
RabMAb

Recombinant Anti-NeuN antibody [EPR21906] - BSA and Azide free (ab239347)

Overview

  • Product name
    Anti-NeuN antibody [EPR21906] - BSA and Azide free
    See all NeuN primary antibodies
  • Description
    Rabbit monoclonal [EPR21906] to NeuN - BSA and Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein within Human NeuN aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: A6NFN3

  • Positive control
    • IHC-P: Human cerebellum tissue.
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).

    ab239347 is a PBS-only buffer format of ab236870. Please refer to ab236870 for recommended dilutions, protocols, and image data.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab239347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 34 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.

Perform heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

IP Use at an assay dependent concentration.

Target

Images

  • Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebellum tissue labeling NeuN with ab236870 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic and nuclear staining in mouse cerebellum (PMID:19713214).

    The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236870).

  • Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebellum tissue labeling NeuN with ab236870 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic and nuclear staining in rat cerebellum (PMID:19713214).

    The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236870).

  • NeuN was immunoprecipitated from 0.35 mg human cerebellum lysate with ab236870 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab236870 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Human cerebellum lysate 10 µg (Input).
    Lane 2: ab236870 IP in human cerebellum lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab236870 in human cerebellum lysate (-).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    The molecular weight observed is consistent with what has been described in the literature (PMID:1483388; PMID:26085943).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236870).

  • Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling NeuN with ab236870 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining in neurons of rat cerebrum (PMID:8813082; PMID:1483388; PMID:26085943). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236870).

  • Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling NeuN with ab236870 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining in neurons of mouse cerebrum (PMID:8813082; PMID:1483388; PMID:26085943). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236870).

  • Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling NeuN with ab236870 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining in neurons of human cerebrum (PMID:8813082; PMID:1483388; PMID:26085943). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236870).

  • Immunohistochemical analysis of paraffin-embedded human cerebellum tissue labeling NeuN with ab236870 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining in granule cells of human cerebellum (PMID:8813082; PMID:1483388). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab236870).

References

ab239347 has not yet been referenced specifically in any publications.

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