Anti-Neurocan antibody [650.24] (ab31979)
Key features and details
- Mouse monoclonal [650.24] to Neurocan
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Neurocan antibody [650.24]
See all Neurocan primary antibodies -
Description
Mouse monoclonal [650.24] to Neurocan -
Host species
Mouse -
Specificity
Specific for Neurocan core protein; also reacts with neurocan C fragment -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Tissue, cells or virus corresponding to Rat Neurocan.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
650.24 -
Myeloma
P3x63 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab31979 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (4) |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
Target
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Function
May modulate neuronal adhesion and neurite growth during development by binding to neural cell adhesion molecules (NG-CAM and N-CAM). Chondroitin sulfate proteoglycan; binds to hyaluronic acid. -
Tissue specificity
Brain. -
Sequence similarities
Belongs to the aggrecan/versican proteoglycan family.
Contains 1 C-type lectin domain.
Contains 2 EGF-like domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Contains 2 Link domains.
Contains 1 Sushi (CCP/SCR) domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 1463 Human
- Entrez Gene: 13004 Mouse
- Entrez Gene: 58982 Rat
- Omim: 600826 Human
- SwissProt: O14594 Human
- SwissProt: P55066 Mouse
- SwissProt: P55067 Rat
- Unigene: 169047 Human
see all -
Alternative names
- Chondroitin sulfate proteoglycan 3 antibody
- Cspg3 antibody
- Ncan antibody
see all
Images
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ab31979 staining Neurocan in rat glioma cell line C6 by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton X-100, blocked with 0.5% BSA for 20 minutes at room temperature, then incubated with ab31979 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a TRITC conjugated goat anti-mouse polyclonal, used at a 1/300 dilution. Nuclei are counterstained with DAPI.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab31979 has been referenced in 3 publications.
- Su H et al. TMAO Aggregates Neurological Damage Following Ischemic Stroke by Promoting Reactive Astrocytosis and Glial Scar Formation via the Smurf2/ALK5 Axis. Front Cell Neurosci 15:569424 (2021). PubMed: 33815059
- Huang X et al. Overexpression of the transcription factors OCT4 and KLF4 improves motor function after spinal cord injury. CNS Neurosci Ther N/A:N/A (2020). PubMed: 32449258
- Li G et al. Mdivi-1 Inhibits Astrocyte Activation and Astroglial Scar Formation and Enhances Axonal Regeneration after Spinal Cord Injury in Rats. Front Cell Neurosci 10:241 (2016). WB, ICC/IF ; Rat . PubMed: 27807407