Anti-Neutrophil Elastase antibody (ab68672)
Key features and details
- Rabbit polyclonal to Neutrophil Elastase
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-Neutrophil Elastase antibody
See all Neutrophil Elastase primary antibodies -
Description
Rabbit polyclonal to Neutrophil Elastase -
Host species
Rabbit -
Specificity
We have had mixed results for use of this antibody in IHC-P using Mouse tissue samples. The abReview data below from external researchers reports good staining with this antibody in Mouse tissue samples using IHC-Fr. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Horse, Dog
-
Immunogen
Synthetic peptide corresponding to Human Neutrophil Elastase aa 1-100 (internal sequence) conjugated to keyhole limpet haemocyanin.
(Peptide available asab68671) -
Positive control
- WB: Mouse Bone Marrow, U-937 Whole Cell Lysate, Native human Neutrophil Elastase protein. IHC-P: Human spleen.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Assay kits
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab68672 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 29 kDa). In our hands, when tested in western blot, this product typically gives a weaker signal in human tissue lysates compared to human cell lines and mouse lysates. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above. |
|
| IHC-P | 1/100 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
-
Function
Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis. -
Tissue specificity
Bone marrow cells. -
Involvement in disease
Defects in ELANE are a cause of cyclic haematopoiesis (CH) [MIM:162800]; also known as cyclic neutropenia. CH is an autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Circulating neutrophils vary between almost normal numbers and zero. During intervals of neutropenia, affected individuals are at risk for opportunistic infection. Monocytes, platelets, lymphocytes and reticulocytes also cycle with the same frequency.
Defects in ELANE are the cause of neutropenia severe congenital autosomal dominant type 1 (SCN1) [MIM:202700]. SCN1 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections. -
Sequence similarities
Belongs to the peptidase S1 family. Elastase subfamily.
Contains 1 peptidase S1 domain. - Information by UniProt
-
Database links
- Entrez Gene: 1991 Human
- Entrez Gene: 50701 Mouse
- Omim: 130130 Human
- SwissProt: P08246 Human
- SwissProt: Q3UP87 Mouse
- Unigene: 99863 Human
- Unigene: 262194 Mouse
-
Alternative names
- Bone marrow serine protease antibody
- ELA2 antibody
- ELANE antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neutrophil Elastase antibody (ab68672)
IHC image of Neutrophil Elastase staining in human spleen formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab68672, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
-
Western blot - Anti-Neutrophil Elastase antibody (ab68672)All lanes : Anti-Neutrophil Elastase antibody (ab68672) at 1 µg/ml
Lane 1 : Bone Marrow (Human) Tissue Lysate - adult normal tissue at 20 µg
Lane 2 : Mouse Bone Marrow Tissue Lysate at 20 µg
Lane 3 : HepG2 Whole Cell Lysate (Negative Control) at 10 µg
Lane 4 : U-937 Whole Cell Lysate at 10 µg
Lane 5 : U-937 (PMA Treated) Whole Cell Lysate (Negative Control)
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab68672 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neutrophil Elastase antibody (ab68672)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United KingdomImmunohistochemistical detection of Neutrophil Elastase using antibody (ab68672) on formaldehyde-fixed paraffin-embedded human spleen sections. Antigen retrieval step: heat mediated in citric acid pH6 buffer. Permeabilization: No Blocking step: 1% BSA for 10 mins @ rt°C. Primary antibody dilution 1/2000; incubation time: 2 hours in TBS/BSA/azide. Secondary Antibody: anti Rabbit IgG conjugated to biotin (1/200).
-
Western blot - Anti-Neutrophil Elastase antibody (ab68672)Anti-Neutrophil Elastase antibody (ab68672) at 1 µg/ml +Native human Neutrophil Elastase protein (Active) (ab91099) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Exposure time: 2 minutes
Protocols
References (91)
ab68672 has been referenced in 91 publications.
- de Bont CM et al. Neutrophil proteases degrade autoepitopes of NET-associated proteins. Clin Exp Immunol 199:1-8 (2020). PubMed: 31661552
- Chauhan A et al. The platelet receptor CLEC-2 blocks neutrophil mediated hepatic recovery in acetaminophen induced acute liver failure. Nat Commun 11:1939 (2020). PubMed: 32321925
- Singh BB et al. Decrease in alpha-1 antiproteinase antitrypsin is observed in primary Sjogren's syndrome condition. Autoimmunity N/A:1-13 (2020). PubMed: 32449389
- Segoviano-Ramirez JC et al. The Diethylcarbamazine Delays and Decreases the NETosis of Polymorphonuclear Cells of Humans with DM Type 2. J Diabetes Res 2020:4827641 (2020). PubMed: 32190698
- Costanzo-Garvey DL et al. Neutrophils are mediators of metastatic prostate cancer progression in bone. Cancer Immunol Immunother 69:1113-1130 (2020). PubMed: 32114681
