Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody (ab151580)
Key features and details
- Rabbit polyclonal to Nicotinic Acetylcholine Receptor alpha 3/CHRNA3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody
See all Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 primary antibodies -
Description
Rabbit polyclonal to Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Molt-4 cell lysate; A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151580 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 57 kDa.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 57 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. -
Sequence similarities
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-3/CHRNA3 sub-subfamily. -
Cellular localization
Cell junction > synapse > postsynaptic cell membrane. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 386578 Chicken
- Entrez Gene: 282702 Cow
- Entrez Gene: 1136 Human
- Entrez Gene: 110834 Mouse
- Entrez Gene: 25101 Rat
- Omim: 118503 Human
- SwissProt: P09481 Chicken
- SwissProt: Q07263 Cow
see all -
Alternative names
- ACHA3_HUMAN antibody
- AChR antibody
- Cholinergic receptor neuronal nicotinic alpha polypeptide 3 antibody
see all
Images
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Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody (ab151580) at 1/1000 dilution + Molt-4 cell lysate at 30 µg
Predicted band size: 57 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of methanol-fixed A431 cells labeling Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 with ab151580 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab151580 has not yet been referenced specifically in any publications.