Anti-Nitrotyrosine antibody (ab42789)
Key features and details
- Rabbit polyclonal to Nitrotyrosine
- Suitable for: WB, ELISA, IHC-FoFr, IHC-P
- Isotype: IgG
Overview
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Product name
Anti-Nitrotyrosine antibody
See all Nitrotyrosine primary antibodies -
Description
Rabbit polyclonal to Nitrotyrosine -
Host species
Rabbit -
Specificity
This antibody reacts with free and bound nitrotyrosine. There is no cross reaction with free or bound tyrosine -
Tested applications
Suitable for: WB, ELISA, IHC-FoFr, IHC-Pmore details -
Species reactivity
not applicable -
Immunogen
Chemical/ Small Molecule corresponding to Nitrotyrosine conjugated to keyhole limpet haemocyanin.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab42789 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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IHC-FoFr |
Use at an assay dependent concentration. PubMed: 22540017
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IHC-P |
Use at an assay dependent concentration. PubMed: 24551153
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Notes |
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WB
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
IHC-FoFr
Use at an assay dependent concentration. PubMed: 22540017 |
IHC-P
Use at an assay dependent concentration. PubMed: 24551153 |
Target
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Relevance
The cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected, estimated semi-quantitatively and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten.
Datasheets and documents
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Datasheet download
References (21)
ab42789 has been referenced in 21 publications.
- Wang Y et al. Slow flow induces endothelial dysfunction by regulating thioredoxin-interacting protein-mediated oxidative metabolism and vascular inflammation. Front Cardiovasc Med 9:1064375 (2022). PubMed: 36465470
- Patel B et al. Inhibition of mitochondrial fission and iNOS in the dorsal vagal complex protects from overeating and weight gain. Mol Metab 43:101123 (2021). PubMed: 33227495
- Török M et al. Network analysis of the left anterior descending coronary arteries in swim-trained rats by an in situ video microscopic technique. Biol Sex Differ 12:37 (2021). PubMed: 34039432
- Musicki B et al. TSPO ligand FGIN-1-27 controls priapism in sickle cell mice via endogenous testosterone production. J Cell Physiol 236:3073-3082 (2021). PubMed: 32974910
- Ryan BJ et al. REST Protects Dopaminergic Neurons from Mitochondrial and α-Synuclein Oligomer Pathology in an Alpha Synuclein Overexpressing BAC-Transgenic Mouse Model. J Neurosci 41:3731-3746 (2021). PubMed: 33563726